An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry

TRNKOVÁ, Libuše, František JELEN, Zdeňka BALCAROVÁ and Kamila NEPLECHOVÁ. An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry. In The 61st Annual Meeting of the International Society of Electrochemistry, the book of abstracts. 2010. ISBN 978-80-7375-396-2.

Basic information

• Original name: An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry
• Authors: TRNKOVÁ, Libuše (203 Czech Republic, guarantor, belonging to the institution), František JELEN (203 Czech Republic), Zdeňka BALCAROVÁ (203 Czech Republic, belonging to the institution) and Kamila NEPLECHOVÁ (203 Czech Republic, belonging to the institution).
• Edition: The 61st Annual Meeting of the International Society of Electrochemistry, the book of abstracts, 2010.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10403 Physical chemistry
• Country of publisher: France
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00051671
• Organization unit: Faculty of Science
• ISBN: 978-80-7375-396-2
• Keywords in English: oligonucleotides;EVLS;heptamer;adenine;cytosine
• Tags: AKb, rivok

Abstract

Short oligonucleotides play an important role in many biological processes including triplet repeat expansion associated with neurodegenerative diseases (fragile X syndrome, Huntington’s disease, Friedreich’s ataxia or myoclonic epilepsy) [1]. One of the most stable DNA fragments, which forms hairpin, is heptamer d(GCGAAGC) [2]. In our electrochemical study we analyzed not only this hairpin but also DNA and RNA fragments containing self-complementary nucleobases (GC) forming the stem. Both DNA and RNA analogues adsorbed on mercury electrodes provided voltammetric reduction signals of adenine and cytosine (A and C) and oxidation signals of guanine (G). We found significant differences between DNA and RNA fragments depending on their length, sequence, and pH (Fig. 1). For the resolution of A and C reduction signals and evaluation of the nature of anodic processes of the G signal in both DNA and RNA fragments, elimination voltammetry with linear scan (EVLS) was utilized [3,4].

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)