Detailed Information on Publication Record
2011
Gene expression profiling in follicular lymphoma and its implication for clinical practise
JANÍKOVÁ, Andrea, Boris TICHÝ, Jana ŠUPÍKOVÁ, Kateřina STAŇO KOZUBÍK, Šárka POSPÍŠILOVÁ et. al.Basic information
Original name
Gene expression profiling in follicular lymphoma and its implication for clinical practise
Name in Czech
Profilování genové exprese a jeho důsledky pro klinickou praxi
Authors
JANÍKOVÁ, Andrea (203 Czech Republic, guarantor), Boris TICHÝ (203 Czech Republic, belonging to the institution), Jana ŠUPÍKOVÁ (203 Czech Republic, belonging to the institution), Kateřina STAŇO KOZUBÍK (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Leoš KŘEN (203 Czech Republic, belonging to the institution), Ingrid VÁŠOVÁ (203 Czech Republic), David ŠÁLEK (203 Czech Republic) and Jiří MAYER (203 Czech Republic)
Edition
LEUKEMIA & LYMPHOMA, London, Informa Healthcare / Leonidas Platanias, 2011, 1042-8194
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.580
RIV identification code
RIV/00216224:14740/11:00050715
Organization unit
Central European Institute of Technology
UT WoS
000286901600012
Keywords in English
follicular lymphoma; gene expression profiling; lymphomagenesis; prognosis
Tags
International impact
Změněno: 25/3/2012 07:09, Olga Křížová
V originále
Follicular lymphoma(FL) is characterized by an indolent and relapsing course. Recently, the clinical outcome of FL has been distinguished by immune microenvironment associated gene signatures. In our study, gene expression profiling (GEP) was performed in 31 nonselected patients with follicular lymphoma (FL), 12 of whom were in relapse and the remaining 19 newly diagnosed. A custom oligonucleotide microarray (Agilent 8 x 15K) was used which contained probes for about 3500 genes, including those that had been previously published as demonstrating significant prognostic value. An unsupervised approach was not able to recognize clinically different FLs. As the previously published prognostically relevant gene signatures could not be properly verified, probably due to microarray platform differences, template matching was therefore used in order to define two gene sets with differential gene expression among our samples. These gene sets shared an overrepresentation of genes with similar biological functions and were termed T CELL and PROLIFERATION profiles. The poor profile was then defined by a high PROLIFERATION score (upper tertile) and or low T CELL score (lower tertile). The poor profile cohort contained a significantly higher proportion of relapsed cases (p 0,05 Fishers exact test). Additionally, a comparison of samples from initial diagnosis and from relapse showed significant differences mainly in the T CELL profile (p=0,036 x (2)). This supports the hypothesis that the number of T cells and their expression pattern play a major role in FL development.
In Czech
Folikulární lymfom (FL) je charakteristický svým indolentním či relabujícím průběhem. V současnosti je možné rozlišit klinický průběh FL pomocí profilování genové exprese v závislosti na mikroprostředí FL. V naší studii jsme stanovili profil genové exprese u 31 pacientů s FL (12 v relapsu, 19 nově diagnostikovaných). K analýze expresního profilu pacientů jsme použili tzv. custom-array (Agilent, formát 8x15K). Unsupervised analýza neodhlalila žádné statisticky významné rozdíly, za použití jiného statistického přístupu (tzv. template matching algorithm) jsme rozlišili dvě sady genů se společnou biologickou funkcí (T-CELL a PROLIFERATION), jejichž exprese se u jednotlivých pacientů lišila v závislosti na klinických projevech onemocnění. Naše práce podporuje hypotézu, že množství T-buněk a jejich expresní profil hraje ve vývoji FL klíčovou roli.
Links
| FR-TI2/254, research and development project |
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| MSM0021622430, plan (intention) |
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