| SLANÝ, Michal, Vanerkova MARTINA, Eva NĚMCOVÁ, Barbora ŽALOUDÍKOVÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis. Canadian Journal of Microbiology. Ottawa, Canada: N R C Research Press, 2010, roč. 56, č. 12, s. 1040-1049. ISSN 0008-4166. Dostupné z: https://dx.doi.org/10.1139/W10-091. |
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@article{919905,
author = {Slaný, Michal and Martina, Vanerkova and Němcová, Eva and Žaloudíková, Barbora and Růžička, Filip and Freiberger, Tomáš},
article_location = {Ottawa, Canada},
article_number = {12},
doi = {http://dx.doi.org/10.1139/W10-091},
keywords = {HRMA; real-time PCR; 16S rRNA; bacterial detection},
language = {eng},
issn = {0008-4166},
journal = {Canadian Journal of Microbiology},
title = {Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis},
volume = {56},
year = {2010}
}
TY - JOUR
ID - 919905
AU - Slaný, Michal - Martina, Vanerkova - Němcová, Eva - Žaloudíková, Barbora - Růžička, Filip - Freiberger, Tomáš
PY - 2010
TI - Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis
JF - Canadian Journal of Microbiology
VL - 56
IS - 12
SP - 1040-1049
EP - 1040-1049
PB - N R C Research Press
SN - 00084166
KW - HRMA
KW - real-time PCR
KW - 16S rRNA
KW - bacterial detection
N2 - High-resolution melting analysis (HRMA) is a fast high-throughput method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range PCR within the 16S rRNA gene where sequence differences are minimal. Genomic DNA samples isolated from 12 reference strains were subjected to a real-time PCR amplification of the 16S rRNA gene in the presence of fluorescent dye, followed by HRMA. Melting profiles were used as molecular fingerprints for bacterial species differentiation. HRMA of S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unrelated strain.
ER -
SLANÝ, Michal, Vanerkova MARTINA, Eva NĚMCOVÁ, Barbora ŽALOUDÍKOVÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Differentiation of Staphylococcus spp. by High-Resolution Melting Analysis. \textit{Canadian Journal of Microbiology}. Ottawa, Canada: N R C Research Press, 2010, roč.~56, č.~12, s.~1040-1049. ISSN~0008-4166. Dostupné z: https://dx.doi.org/10.1139/W10-091.
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