VLKOVÁ, Marcela, E. FROŇKOVÁ, V. KANDEROVÁ, A. JANDA, Š. RŮŽIČKOVÁ, Jiří LITZMAN, A. ŠEDIVÁ and T. KALINA. Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. Journal of immunology. Bethesda: American association of immunologists, 2010, vol. 185, No 11, p. 6431-6438. ISSN 0022-1767. doi:10.4049/jimmunol.0903876.
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Basic information
Original name Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression
Name in Czech Charakterizace lymfocytárních subpopulací u pacientů s CVID rozděluje subpopulace naivních lidských B lymfkocytů na základě exprese znaku CD24
Authors VLKOVÁ, Marcela (203 Czech Republic, guarantor, belonging to the institution), E. FROŇKOVÁ (203 Czech Republic), V. KANDEROVÁ (203 Czech Republic), A. JANDA (203 Czech Republic), Š. RŮŽIČKOVÁ (203 Czech Republic), Jiří LITZMAN (203 Czech Republic, belonging to the institution), A. ŠEDIVÁ (203 Czech Republic) and T. KALINA (203 Czech Republic).
Edition Journal of immunology, Bethesda, American association of immunologists, 2010, 0022-1767.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.745
RIV identification code RIV/00216224:14110/10:00058956
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.4049/jimmunol.0903876
UT WoS 000284311500007
Keywords (in Czech) B lymfocyty; průtokový cytometrie; CVID
Keywords in English B cells; flow cytometry; CVID
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 4/10/2012 14:06.
Abstract
Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID 21norm) or increased (CVID 21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID 21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression.
Abstract (in Czech)
Při studiu B lymfocytárních subpopulací u pacientů s CVID byly nalezeny nové subpopulace naivních B lymfocytů na základě exprese znaků CD24 a povrchového IgM. Tyto subpopulace byly poté nalezeny i u všech kontrolních osob.
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