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@article{920883,
author = {Vlková, Marcela and Froňková, E. and Kanderová, V. and Janda, A. and Růžičková, Š. and Litzman, Jiří and Šedivá, A. and Kalina, T.},
article_location = {Bethesda},
article_number = {11},
doi = {http://dx.doi.org/10.4049/jimmunol.0903876},
keywords = {B cells; flow cytometry; CVID},
language = {eng},
issn = {0022-1767},
journal = {Journal of immunology},
title = {Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression},
volume = {185},
year = {2010}
}
TY - JOUR
ID - 920883
AU - Vlková, Marcela - Froňková, E. - Kanderová, V. - Janda, A. - Růžičková, Š. - Litzman, Jiří - Šedivá, A. - Kalina, T.
PY - 2010
TI - Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression
JF - Journal of immunology
VL - 185
IS - 11
SP - 6431-6438
EP - 6431-6438
PB - American association of immunologists
SN - 00221767
KW - B cells
KW - flow cytometry
KW - CVID
N2 - Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID 21norm) or increased (CVID 21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID 21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression.
ER -
VLKOVÁ, Marcela, E. FROŇKOVÁ, V. KANDEROVÁ, A. JANDA, Š. RŮŽIČKOVÁ, Jiří LITZMAN, A. ŠEDIVÁ and T. KALINA. Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. Online. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2010, vol.~185, No~11, p.~6431-6438. ISSN~0022-1767. Available from: https://dx.doi.org/10.4049/jimmunol.0903876. [citováno 2024-04-24]
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