Celogenomové restrikční mapování u 8 zástupců rodu Treponema.

MIKALOVÁ, Lenka and David ŠMAJS. Celogenomové restrikční mapování u 8 zástupců rodu Treponema. In XIX. Tomáškovy dny. 2010.

Basic information

• Original name: Celogenomové restrikční mapování u 8 zástupců rodu Treponema.
• Authors: MIKALOVÁ, Lenka and David ŠMAJS.
• Edition: XIX. Tomáškovy dny, 2010.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords (in Czech): Treponema pallidum subsp. pallidum; Treponema pallidum subsp. pertenue; Treponema pallidum subsp. endemicum; syfilis; yaws; endemická syfilis; Fribourg-Blanc

Abstract (in English)

Introduction The genus Treponema includes some species and subspecies pathogenic for humans. Treponema pallidum subsp. pallidum (TPA) causes sexually transmitted disease syphilis, T. p. pertenue (TPE) and T. p. endemicum (TEN) are the causative agents of nonveneral diseases yaws and endemic syphilis. These diseases are typical for areas with humid and dry climate, respectively. The different degree of invasiveness and pathogenicity of these spirochetes reflects differences in treponeme genomes. Methods Eight Treponema pallidum strains were compared by whole genome fingerprinting (WGF). We used 4 TPA strains – Nichols, DAL-1, Mexico A, SS14; 3 TPE strains - Samoa D, CDC-2, Gauthier and one unclassified strain Fribourg-Blanc isolated from baboon. Results Based on WGF technique, genome sizes and genetic relatedness of studied treponemal strains were estimated. Although TPA and TPE strains are highly similar, it is possible to discriminate between them in 6 genomic regions, where 4 deletions (33 – 377bp) and 2 insertions (52bp and 377bp) were discovered. The simian strain Fribourg-Blanc was similar to TPE strains suggesting its relatedness to TPE strains. Genes TP0433-434 and TP0470 contained repetitive sequence with strain specific number of repetitions. Five strains (Nichols, Samoa D, DAL-1, Gauthier and Fribourg-Blanc) showed unique genome differences, which can be useful in diagnostics and epidemiology. Most of the observed differences were localized in tpr loci and in the vicinity of these loci, suggesting their possible role in the pathogenicity of Treponema pallidum.

Links

• GA310/07/0321, research and development project: Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• NR8967, research and development project: Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministry of Health of the CR