Jednoduchá metoda k průkazu CD154 (CD40L) na lymfocytech v periferní krvi

THON, Vojtěch, Marcela VLKOVÁ, Jiří LITZMAN and Jindřich LOKAJ. Jednoduchá metoda k průkazu CD154 (CD40L) na lymfocytech v periferní krvi (A simple method for the detection of CD154 (CD40L) on peripheral blood lymphocytes). Online. Epidemiologie Mikrobiologie Imunologie. 2010, vol. 59, No 3, p. 147-154. ISSN 1210-7913. [citováno 2024-04-23]

Basic information

• Original name: Jednoduchá metoda k průkazu CD154 (CD40L) na lymfocytech v periferní krvi
• Name in Czech: Jednoduchá metoda k průkazu CD154 (CD40L) na lymfocytech v periferní krvi
• Name (in English): A simple method for the detection of CD154 (CD40L) on peripheral blood lymphocytes
• Authors: THON, Vojtěch (203 Czech Republic, guarantor, belonging to the institution), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution), Jiří LITZMAN (203 Czech Republic, belonging to the institution) and Jindřich LOKAJ (203 Czech Republic, belonging to the institution)
• Edition: Epidemiologie Mikrobiologie Imunologie, 2010, 1210-7913.

Other information

• Original language: Czech
• Type of outcome: Article in a journal
• Field of Study: 30102 Immunology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/10:00058964
• Organization unit: Faculty of Medicine
• UT WoS: 000208443500008
• Keywords (in Czech): CD154; CD40L; CD40; syndrom hyperimunoglobulinémie M; průtoková cytometrie
• Keywords in English: CD154; CD40L; CD40; hyper-IgM syndrome; flow cytometry
• Tags: International impact, Reviewed

Abstract

Molekula CD154 (CD40L) je transmembránový glykoprotein přítomný především v aktivovaných lymfocytech T CD4+. Jeho receptorem je molekula CD40, která je v membráně lymfocytů B, ale též jiných buněk. Interakce CD154 CD40 je podmínkou optimálního rozvoje adaptivní imunitní reakce, jejím důsledkem je však i ovlivnění zánětlivého procesu. Porucha funkce CD154 je patognomická pro závažnou primární imunodeficienci syndrom hyperimunoglobulinemie M (XHIGM). Odhalení abnormality CD154 je základem pro stanovení diagnózy tohoto syndromu. Naše metodika k průkazu CD154 na lymfocytech aktivovaných v plné krvi je časově nenáročná, vyžaduje malé množství (0,5 ml) krve a lze ji doporučit při vstupním vyšetření dětí s podezřením na syndrom hyperimunoglobulinemie M. Umožňuje také záchyt přenašeček XHIGM.

Abstract (in English)

CD154 (also called CD40L) is a transmembrane glycoprotein predominantly expressed on the surface membrane of activated CD4+ T cells. Its receptor CD40 is present on all B cells, but also on other cells. The interaction CD154-CD40 is necessary for the optimal development of the adaptive immune response and also has consequences for the modulation of the inflammatory response. A defect in the expression of CD154 is pathognomonic of congenital immunodeficiency called X-linked Hyper-IgM syndrome (XHIGM). To detect the abnormality of CD154 is essential for making the diagnosis of XHIGM. The presented microtest for the detection of CD154 on activated lymphocytes in whole blood is fast and blood saving, since as little as 0.5 ml of blood is needed to complete it. It can be recommended as the initial test for suspected hyper-IgM syndrome in children. We demonstrate that this screening method can help to detect also carriers of XHIGM.

Links

• 7E08062, research and development project: Name: Pathophysiology and Natural Course of Primary Antibody Deficiency (PAD) Syndromes

Investor: Ministry of Education, Youth and Sports of the CR, Pathophysiology and Natural Course of Primary Antibody Deficiency (PAD) Syndromes