HÝŽA, Petr, Libor STREIT, Eduard GOPFERT, Daniel SCHWARZ, Michal MASAŘÍK, Michal JURAJDA, Anna VAŠKŮ and Jiří VESELÝ. Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model. ACTA VETERINARIA BRNO. VFU Brno: VFU Brno, 2010, vol. 79, No 3, p. 453-457. ISSN 0001-7213.
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Basic information
Original name Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
Authors HÝŽA, Petr (203 Czech Republic, guarantor, belonging to the institution), Libor STREIT (203 Czech Republic, belonging to the institution), Eduard GOPFERT (203 Czech Republic), Daniel SCHWARZ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Michal JURAJDA (203 Czech Republic, belonging to the institution), Anna VAŠKŮ (203 Czech Republic, belonging to the institution) and Jiří VESELÝ (203 Czech Republic, belonging to the institution).
Edition ACTA VETERINARIA BRNO, VFU Brno, VFU Brno, 2010, 0001-7213.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.534
RIV identification code RIV/00216224:14110/10:00040874
Organization unit Faculty of Medicine
UT WoS 000285269100016
Keywords in English Vasospasm; pathogenesis; microsurgery; pig
Changed by Changed by: MUDr. Michal Jurajda, Ph.D., učo 24045. Changed: 21/1/2011 11:03.
Abstract
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
Abstract (in Czech)
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
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NR8368, research and development projectName: Spazmy cév mikrochirurgicky přenášených laloků a možnosti jejich ovlivnění
Investor: Ministry of Health of the CR
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