Detailed Information on Publication Record
2010
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
HÝŽA, Petr, Libor STREIT, Eduard GOPFERT, Daniel SCHWARZ, Michal MASAŘÍK et. al.Basic information
Original name
Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model
Authors
HÝŽA, Petr (203 Czech Republic, guarantor, belonging to the institution), Libor STREIT (203 Czech Republic, belonging to the institution), Eduard GOPFERT (203 Czech Republic), Daniel SCHWARZ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Michal JURAJDA (203 Czech Republic, belonging to the institution), Anna VAŠKŮ (203 Czech Republic, belonging to the institution) and Jiří VESELÝ (203 Czech Republic, belonging to the institution)
Edition
ACTA VETERINARIA BRNO, VFU Brno, VFU Brno, 2010, 0001-7213
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.534
RIV identification code
RIV/00216224:14110/10:00040874
Organization unit
Faculty of Medicine
UT WoS
000285269100016
Keywords in English
Vasospasm; pathogenesis; microsurgery; pig
Změněno: 21/1/2011 11:03, MUDr. Michal Jurajda, Ph.D.
V originále
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
In Czech
The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 was not proven
Links
| NR8368, research and development project |
|