MARTIN, Harry, Elisabeth BURGESS, Michal MASAŘÍK, Karl KRAMER, Miroslava BEKLOVÁ, Vojtěch ADAM and René KIZEK. Avidin and plant biotechnology to control pests. In Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming. 1st Edition. New York: Springer, 2010, p. 1-22. Sustainable Agriculture Reviews Volume 4. ISBN 978-90-481-8740-9.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Avidin and plant biotechnology to control pests
Authors MARTIN, Harry (840 United States of America), Elisabeth BURGESS (36 Australia), Michal MASAŘÍK (203 Czech Republic), Karl KRAMER (840 United States of America), Miroslava BEKLOVÁ (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic, belonging to the institution) and René KIZEK (203 Czech Republic, guarantor, belonging to the institution).
Edition 1st Edition. New York, Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming, p. 1-22, 22 pp. Sustainable Agriculture Reviews Volume 4, 2010.
Publisher Springer
Other information
Original language English
Type of outcome Chapter(s) of a specialized book
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/10:00051778
Organization unit Faculty of Medicine
ISBN 978-90-481-8740-9
Keywords in English Transgenic plants; avidinbiotin technology; agriculture; electrochemical method
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 5/4/2012 16:12.
Abstract
In this review, we discuss the application of transgenic avidin, a protein naturally occurring in eggwhite, in the protection of rice, maize, potato and apple leaf from insect pests. Avidin binds the vitamin, biotin with extraordinary affinity (10minus15 M). Biotin is a watersoluble vitamin that is required for normal cellular metabolism and growth. The presence of avidin in the diet of insect pests is lethal since biotin is unavailable to them. The use of streptavidin, a bacterial homologue of avidin, is also described. We discuss the subcellular targeting of avidin expression in plants to avoid toxicity to the plant host and we describe the qualities of avidin which make it suitable for crop protection during cultivation and storage. Avidin is stable under normal conditions of crop storage but biodegradable and destroyed by cooking. These combined qualities make it an excellent choice for the protection of crops from insects. Finally, we discuss the modification of the avidin gene to allow expression in plants, the methods for transfection of the gene into plants, and the approaches used to quantify gene expression and avidin function in plant tissues. These methods include: polymerase chain reaction; enzymelinked immmunosorbent assay; polyacrylamide gelelectrophoresis; fluorescence polarisation (FP); capillary electrophoresis; tissueprinting; square-wave voltammetry (SWV) and the measurement of larvae morbidity and mortality.
PrintDisplayed: 1/8/2024 02:22