Increasing hematopoietic microchimerism is a reliable indicator
of incipient AML relapse

J 2010

Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ et. al.

Basic information

Original name

Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse

Authors

HORKÝ, Ondřej (203 Czech Republic, guarantor, belonging to the institution), Jiří MAYER (203 Czech Republic, belonging to the institution), Lenka KABLÁSKOVÁ (203 Czech Republic, belonging to the institution), Filip RÁZGA (703 Slovakia, belonging to the institution), Marta KREJČÍ (203 Czech Republic, belonging to the institution), Jarmila KISSOVÁ (203 Czech Republic, belonging to the institution), Marek BORSKÝ (203 Czech Republic, belonging to the institution), Ivana JEŽÍŠKOVÁ (203 Czech Republic) and Dana DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution)

Edition

International Journal of Laboratory Hematology, 2010, 1751-5521

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.368

RIV identification code

RIV/00216224:14110/10:00047063

Organization unit

Faculty of Medicine

UT WoS

000286052900006

Keywords in English

microchimerism; chimerism; real-time PCR; relapse; acute myeloid leukemia

Změněno: 10/5/2011 08:45, Mgr. Ondřej Horký, Ph.D.

Abstract

V originále

Using real-time PCR (RQ-PCR), we compare peripheral blood samples obtained from patients with acute myeloid leukemia (AML) before hematological relapse and those taken during complete remission. By comparison of these two groups, we describe microchimerism dynamics clearly connected with imminent AML relapse. Mere reappearance of autologous hematopoiesis within patients with complete donor chimerism is alarming, and another sample with further increase confirms ongoing relapse. In case of patients with continuous microchimerism, another two consecutive samples with increasing trend are required. RQ-PCR predicted a significantly higher number of hematological relapses (87% vs. 39%) with a median anticipation period of 33 days, 26 days earlier than conventional PCR (P = 0.0002). Moreover, the outcome of microchimerism dynamics was in complete agreement with monitoring of minimal residual disease when analyzed from the same cell compartment.

Links

MSM0021622430, plan (intention)

Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

Citovat

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ, Jarmila KISSOVÁ, Marek BORSKÝ, Ivana JEŽÍŠKOVÁ and Dana DVOŘÁKOVÁ. Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse. International Journal of Laboratory Hematology. 2010, vol. 33, No 1, 10 pp. ISSN 1751-5521.

@article{922804,
   author = {Horký, Ondřej and Mayer, Jiří and Kablásková, Lenka and Rázga, Filip and Krejčí, Marta and Kissová, Jarmila and Borský, Marek and Ježíšková, Ivana and Dvořáková, Dana},
   article_number = {1},
   keywords = {microchimerism; chimerism; real-time PCR; relapse; acute myeloid leukemia},
   language = {eng},
   issn = {1751-5521},
   journal = {International Journal of Laboratory Hematology},
   title = {Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse},
   volume = {33},
   year = {2010}
}

TY  - JOUR
ID  - 922804
AU  - Horký, Ondřej - Mayer, Jiří - Kablásková, Lenka - Rázga, Filip - Krejčí, Marta - Kissová, Jarmila - Borský, Marek - Ježíšková, Ivana - Dvořáková, Dana
PY  - 2010
TI  - Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse
JF  - International Journal of Laboratory Hematology
VL  - 33
IS  - 1
SN  - 17515521
KW  - microchimerism
KW  - chimerism
KW  - real-time PCR
KW  - relapse
KW  - acute myeloid leukemia
N2  - Using real-time PCR (RQ-PCR), we compare peripheral blood samples obtained from patients with acute myeloid leukemia (AML) before hematological relapse and those taken during complete remission. By comparison of these two groups, we describe microchimerism dynamics clearly connected with imminent AML relapse. Mere reappearance of autologous hematopoiesis within patients with complete donor chimerism is alarming, and another sample with further increase confirms ongoing relapse. In case of patients with continuous microchimerism, another two consecutive samples with increasing trend are required. RQ-PCR predicted a significantly higher number of hematological relapses (87% vs. 39%) with a median anticipation period of 33 days, 26 days earlier than conventional PCR (P = 0.0002). Moreover, the outcome of microchimerism dynamics was in complete agreement with monitoring of minimal residual disease when analyzed from the same cell compartment.
ER  -

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Filip RÁZGA, Marta KREJČÍ, Jarmila KISSOVÁ, Marek BORSKÝ, Ivana JEŽÍŠKOVÁ and Dana DVOŘÁKOVÁ. Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse. \textit{International Journal of Laboratory Hematology}. 2010, vol.~33, No~1, 10 pp. ISSN~1751-5521.

Detailed Information on Publication Record