The Expression of NADPH Oxidases and Production of Reactive
Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell
Line A549

J 2010

The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549

KOLÁŘOVÁ, Hana, Lucia BINÓ, Kateřina PEJCHALOVÁ and Lukáš KUBALA

Basic information

Original name

The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549

Name in Czech

Exprese NADPH oxidáz a produkce reaktivních metabolitů kyslíku lidskou plicní adenokarcinomní epiteliální buněčnou linií A549

Authors

KOLÁŘOVÁ, Hana, Lucia BINÓ, Kateřina PEJCHALOVÁ and Lukáš KUBALA

Edition

Folia biologica, Praha, CHARLES UNIV PRAGUE, FIRST FACULTY MEDIC, 2010, 0015-5500

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.729

Organization unit

Faculty of Science

UT WoS

000284254900004

Keywords in English

HYDROGEN-PEROXIDE; HOST-DEFENSE; NITRIC-OXIDE; SUPEROXIDE; PHAGOCYTES; CHEMILUMINESCENCE; DIFFERENTIATION; L-012

Abstract

ORIG CZ

V originále

Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2’,7’-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.

In Czech

U lidské plicní epiteliální linie A549 byla prokázána exprese NOX1, NOX2, DUOX1 a DUOX2 a detekována produkce ROS pomocí fluorometrických prób 2’,7’-dichlorofluorescein- diacetate, dihydroethidium, and amplex red nebo kolorimetrické próby nitrobluetetrazolium. Tyto výsledky odhalily rozdíly mezi metodami využívanými pro měření produkce ROS buněčnou linií A549 a zvýrazňují citlivost fluorometrického stanovení pro tento účel.

Links

GA524/06/1197, research and development project

Name: Role volných radikálů v regulaci plicního zánětu vyvolaného akutní nebo chronickou expozicí k endotoxinu

GA524/08/1753, research and development project

Name: Vliv L-argininu a jeho analogů na tvorbu reaktivních metabolitů kyslíku a dusíku profesionálními fagocyty

Citovat

KOLÁŘOVÁ, Hana, Lucia BINÓ, Kateřina PEJCHALOVÁ and Lukáš KUBALA. The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549. Folia biologica. Praha: CHARLES UNIV PRAGUE, FIRST FACULTY MEDIC, 2010, vol. 56, No 5, p. 211-7, 7 pp. ISSN 0015-5500.

@article{929254,
   author = {Kolářová, Hana and Binó, Lucia and Pejchalová, Kateřina and Kubala, Lukáš},
   article_location = {Praha},
   article_number = {5},
   keywords = {HYDROGEN-PEROXIDE; HOST-DEFENSE; NITRIC-OXIDE; SUPEROXIDE; PHAGOCYTES; CHEMILUMINESCENCE; DIFFERENTIATION; L-012},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549},
   url = {http://fb.cuni.cz/Data/files/folia_biologica/volume_56_2010_5/FB2010A0029.pdf},
   volume = {56},
   year = {2010}
}

TY  - JOUR
ID  - 929254
AU  - Kolářová, Hana - Binó, Lucia - Pejchalová, Kateřina - Kubala, Lukáš
PY  - 2010
TI  - The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549
JF  - Folia biologica
VL  - 56
IS  - 5
SP  - 211-7
EP  - 211-7
PB  - CHARLES UNIV PRAGUE, FIRST FACULTY MEDIC
SN  - 00155500
KW  - HYDROGEN-PEROXIDE
KW  - HOST-DEFENSE
KW  - NITRIC-OXIDE
KW  - SUPEROXIDE
KW  - PHAGOCYTES
KW  - CHEMILUMINESCENCE
KW  - DIFFERENTIATION
KW  - L-012
UR  - http://fb.cuni.cz/Data/files/folia_biologica/volume_56_2010_5/FB2010A0029.pdf
N2  - Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2’,7’-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.
ER  -

KOLÁŘOVÁ, Hana, Lucia BINÓ, Kateřina PEJCHALOVÁ and Lukáš KUBALA. The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549. \textit{Folia biologica}. Praha: CHARLES UNIV PRAGUE, FIRST FACULTY MEDIC, 2010, vol.~56, No~5, p.~211-7, 7 pp. ISSN~0015-5500.

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