GUMULEC, Jaromír, Michal MASAŘÍK, Marián HLAVNA, Markéta SZTALMACHOVÁ and René KIZEK. Heavy metal status in prostate cancer. In The Student Scientific Conference on Cancer Research. 2011. ISBN 978-80-210-5442-4.
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Basic information
Original name Heavy metal status in prostate cancer
Name in Czech Těžké kovy a karcinom prostay
Authors GUMULEC, Jaromír, Michal MASAŘÍK, Marián HLAVNA, Markéta SZTALMACHOVÁ and René KIZEK.
Edition The Student Scientific Conference on Cancer Research, 2011.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Faculty of Medicine
ISBN 978-80-210-5442-4
Keywords (in Czech) zinc prostate cancer reactive oxygen
Keywords in English zinc prostate cancer reactive oxygen
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 15/7/2011 09:57.
Abstract
Prostate tissue is specific in zinc metabolism – it accumulates high level of zinc(II) – up to ten times higher compared to other tissues. In such concentration, zinc(II) affects energetic metabolism, antioxidative processes and apoptosis. However, prostate cancer has significantly lower intracellular level of zinc(II). Therefore, distinct behaviour of tumor cells is expected. This study focuses on impact of zinc(II) on following cell lines: (1) 22Rv1 (derived from a xenograft from androgen-dependent primary tumor of Gleason sum 9), (2) PNT1A (established by immortalisation of normal adult prostatic epithelial cells by transfection with SV40). Theese cell lines were fed with increasing concentration of zinc(II) in medium. Cell growth was monitored by real-time impedance monitoring system, mRNA and protein level of zinc-related and oxidative-stress-related genes were determined, level of intracellular zinc was deteremined using electrochemical methods. We found significant differences in expression of zinc-transporter genes and zinc-binding proteins between cell lines; we found significantly elevated levels of major zinc transporters in tumorous cell line in mRNA level and correlations between zinc(II) level in medium and level of zinc-binding protein metallothionein in cells. Based on obtained results, it can be concluded that zinc(II) may play a role in prostate cancer pathogenesis.
Abstract (in Czech)
Práce pojednává o vztahu těžkých kovů, zejména zinečnatých iontů a jejich vztahu k patogenezi karcinomu prostaty. Experimenty byly provedeny na buněčných liniích PNT1A a 22Rv1, byl charakterizován růst impedančně a sledovaná exprese zinek-dependentních genů.
Links
GP301/09/P436, research and development projectName: Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu.
Investor: Czech Science Foundation
NS10200, research and development projectName: Nádorové markery, jejich stanovení a korelace s karcinomem prostaty
Investor: Ministry of Health of the CR
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