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@article{935687,
author = {Kostečka, Pavel and Havran, Luděk and Bittová, Miroslava and Pivoňková, Hana and Fojta, Miroslav},
article_number = {1},
keywords = {Single-base mismatch; Nucleobase insertion; Osmium tetroxide; Carbon electrodes; Voltammetry; Chemical probe; Electroactive label},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical Chemistry},
title = {Sensing mispaired thymines in DNA heteroduplexes using an electroactive osmium marker: towards electrochemical SNP probing},
url = {http://www.springerlink.com/content/074k47jthp76x862/},
volume = {400},
year = {2011}
}
TY - JOUR
ID - 935687
AU - Kostečka, Pavel - Havran, Luděk - Bittová, Miroslava - Pivoňková, Hana - Fojta, Miroslav
PY - 2011
TI - Sensing mispaired thymines in DNA heteroduplexes using an electroactive osmium marker: towards electrochemical SNP probing
JF - Analytical and Bioanalytical Chemistry
VL - 400
IS - 1
SP - 197-204
EP - 197-204
SN - 16182642
KW - Single-base mismatch
KW - Nucleobase insertion
KW - Osmium tetroxide
KW - Carbon electrodes
KW - Voltammetry
KW - Chemical probe
KW - Electroactive label
UR - http://www.springerlink.com/content/074k47jthp76x862/
L2 - http://www.springerlink.com/content/074k47jthp76x862/
N2 - A complex OsO4, 2,2'-bipyridine (Os,bipy), has been used for electroactive labeling of biopolymers as well as for probing of nucleic acids and protein structure and interactions. In DNA, Os,bipy forms electrochemically active adducts with pyrimidine nucleobases, exhibiting highly selective modification of thymine residues in single-stranded DNA. Here, we show that modification of rare thymine residues (one thymine among several tens of unreactive purine bases) can easily be detected by means of a simple ex situ voltammetric analysis using carbon electrodes. Based on this remarkable sensitivity of detection, Os,bipy has been used as an electroactive probe for unpaired and/or mismatched thymine residues within DNA heteroduplexes. Site-specific chemical modification of the DNA with the Os,bipy has allowed a clear distinction between perfectly base-paired DNA homoduplexes and mismatched heteroduplexes, as well as discrimination among heteroduplexes containing one or two mispaired thymines, a single thymine insertion, or combination of a mispair and an insertion.
ER -
KOSTEČKA, Pavel, Luděk HAVRAN, Miroslava BITTOVÁ, Hana PIVOŇKOVÁ and Miroslav FOJTA. Sensing mispaired thymines in DNA heteroduplexes using an electroactive osmium marker: towards electrochemical SNP probing. \textit{Analytical and Bioanalytical Chemistry}. 2011, vol.~400, No~1, p.~197-204. ISSN~1618-2642.
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