| KADEŘÁVEK, Pavel, Carl DIEHL, Veronika PAPOUŠKOVÁ, Hana ŠANDEROVÁ, Petr PADRTA, Lukáš ŽÍDEK, Libor KRÁSNÝ, Vladimír SKLENÁŘ and Mikael AKKE. Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping. MATERIALS STRUCTURE. 2011, vol. 18, No 1, p. 3-5. ISSN 1211-5894. |
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@article{935903,
author = {Kadeřávek, Pavel and Diehl, Carl and Papoušková, Veronika and Šanderová, Hana and Padrta, Petr and Žídek, Lukáš and Krásný, Libor and Sklenář, Vladimír and Akke, Mikael},
article_number = {1},
keywords = {NMR; delta subunit; RNA polymerase; spectral density function},
language = {eng},
issn = {1211-5894},
journal = {MATERIALS STRUCTURE},
title = {Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping},
url = {http://www.xray.cz/ms/bul2011-1.htm},
volume = {18},
year = {2011}
}
TY - JOUR
ID - 935903
AU - Kadeřávek, Pavel - Diehl, Carl - Papoušková, Veronika - Šanderová, Hana - Padrta, Petr - Žídek, Lukáš - Krásný, Libor - Sklenář, Vladimír - Akke, Mikael
PY - 2011
TI - Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping
JF - MATERIALS STRUCTURE
VL - 18
IS - 1
SP - 3-5
EP - 3-5
SN - 12115894
KW - NMR
KW - delta subunit
KW - RNA polymerase
KW - spectral density function
UR - http://www.xray.cz/ms/bul2011-1.htm
L2 - http://www.xray.cz/ms/bul2011-1.htm
N2 - RNA polymerases of Gram positive and Gram negative bacteria differ. The subunit composition in Gram positive bacteria includes two additional subunits. One of them is denoted delta-subunit. The structure of delta-subunit from Bacillus subtilis has been solved recently using methods of solution NMR [1] (PDB ID = 2KRC). Here, we extend the information about the structure by the basic characterization of its dynamics studied at two temperatures. The standard relaxation experiments (R1, R2, ssNOE) were performed and data were analyzed using reduced spectral density mapping as the most straightforward approach. The analysis reveals flexible residues in the central part of the sequence. It confirms expected independent stochastic movements in the C-terminal part of the molecule. Results also yield an evidence of a slow conformational exchange of several residues in the well-structured region of the protein.
ER -
KADEŘÁVEK, Pavel, Carl DIEHL, Veronika PAPOUŠKOVÁ, Hana ŠANDEROVÁ, Petr PADRTA, Lukáš ŽÍDEK, Libor KRÁSNÝ, Vladimír SKLENÁŘ and Mikael AKKE. Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping. \textit{MATERIALS STRUCTURE}. 2011, vol.~18, No~1, p.~3-5. ISSN~1211-5894.
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