Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species

ŠEDO, Ondrej, Aleš VORÁČ and Zbyněk ZDRÁHAL. Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species. Online. Systematic and Applied Microbiology. Elsevier, 2011, vol. 34, No 1, p. 30-34. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2010.11.008. [citováno 2024-04-24]

Basic information

• Original name: Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species
• Name in Czech: Optimalizace parametrů MALDI-TOF hmotnostních spekter Acinetobacter sp.
• Authors: ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Aleš VORÁČ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)
• Edition: Systematic and Applied Microbiology, Elsevier, 2011, 0723-2020.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.366
• RIV identification code: RIV/00216224:14740/11:00052199
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.syapm.2010.11.008
• UT WoS: 000289395200005
• Keywords (in Czech): Acinetobacter sp.; identifikace bakterií; MALDI-TOF MS
• Keywords in English: Acinetobacter sp.; bacteria identification; MALDI-TOF MS
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

The influence of the matrix solution, sample form and deposition technique on the quality MALDI-TOF mass spectra was examined and assessed with the aim to improve MALDI-TOF MS performance for the identification of microorganisms and to enable automatic spectra acquisition. It was observed that the use of matrix compounds ferulic and sinapinic acid may result in improved mass spectral features, in terms of signal resolution and S/N ratio, as compared to alpha-cyano-4-hydroxycinnamic acid, which was, on the other hand, found to be the only matrix compound that enabled fully automatic mass spectra acquisition. The robustness of the whole sample preparation procedure was then assessed on a set of 25 strains of four Acinetobacter species. Results showed reproducible detection of subtle mass spectral differences between strains belonging to the same species, although they do not confirm the possibility of reliable strain typing.

Abstract (in Czech)

Vliv složení roztoku matrice, formy vzorku a metody nanášení vzorku na kvalitu MALDI-TOF hmotnostních spekter byl zkoumán s ohledem na zlepšení a automatizovatelnost metody. Bylo zjištěno, že použití kyselin ferulové nebo sinapové jako matricí může zlepšit rozlišení a poměr signál/šum, kyselina alfa-kyano-4-hydroxyskořicová oproti tomu byla jedinou matricí, která poskytla hmotnostní spektra automatickým sběrem dat. Robustnost celého postupu byla testována na 25 kmenech Acinetobacter sp. Pozorována byla možnost reprodukovatelně detekovat jemné rozdíly mezi spektry, nicméně nebyla potvrzena možnost spolehlivé klasifikace na kmenové úrovni.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• QI101A094, research and development project: Name: Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza

Investor: Ministry of Agriculture of the CR, Sustainable development of agrarian sector

• 2B08060, research and development project: Name: Vývoj diagnostiky a léčby závažných onemocnění srdce a cév pomocí genomických a epigenomických přístupů (Acronym: EPICARD)

Investor: Ministry of Education, Youth and Sports of the CR, Development of diagnostics and treatment of serious heart and vascular diseases using genomic and proteomic approaches