Detailed Information on Publication Record
2011
Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species
ŠEDO, Ondrej, Aleš VORÁČ and Zbyněk ZDRÁHALBasic information
Original name
Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species
Name in Czech
Optimalizace parametrů MALDI-TOF hmotnostních spekter Acinetobacter sp.
Authors
ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Aleš VORÁČ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Systematic and Applied Microbiology, Elsevier, 2011, 0723-2020
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.366
RIV identification code
RIV/00216224:14740/11:00052199
Organization unit
Central European Institute of Technology
UT WoS
000289395200005
Keywords (in Czech)
Acinetobacter sp.; identifikace bakterií; MALDI-TOF MS
Keywords in English
Acinetobacter sp.; bacteria identification; MALDI-TOF MS
Tags
International impact, Reviewed
Změněno: 7/4/2012 05:49, Olga Křížová
V originále
The influence of the matrix solution, sample form and deposition technique on the quality MALDI-TOF mass spectra was examined and assessed with the aim to improve MALDI-TOF MS performance for the identification of microorganisms and to enable automatic spectra acquisition. It was observed that the use of matrix compounds ferulic and sinapinic acid may result in improved mass spectral features, in terms of signal resolution and S/N ratio, as compared to alpha-cyano-4-hydroxycinnamic acid, which was, on the other hand, found to be the only matrix compound that enabled fully automatic mass spectra acquisition. The robustness of the whole sample preparation procedure was then assessed on a set of 25 strains of four Acinetobacter species. Results showed reproducible detection of subtle mass spectral differences between strains belonging to the same species, although they do not confirm the possibility of reliable strain typing.
In Czech
Vliv složení roztoku matrice, formy vzorku a metody nanášení vzorku na kvalitu MALDI-TOF hmotnostních spekter byl zkoumán s ohledem na zlepšení a automatizovatelnost metody. Bylo zjištěno, že použití kyselin ferulové nebo sinapové jako matricí může zlepšit rozlišení a poměr signál/šum, kyselina alfa-kyano-4-hydroxyskořicová oproti tomu byla jedinou matricí, která poskytla hmotnostní spektra automatickým sběrem dat. Robustnost celého postupu byla testována na 25 kmenech Acinetobacter sp. Pozorována byla možnost reprodukovatelně detekovat jemné rozdíly mezi spektry, nicméně nebyla potvrzena možnost spolehlivé klasifikace na kmenové úrovni.
Links
| LC06034, research and development project |
| ||
| MSM0021622415, plan (intention) |
| ||
| QI101A094, research and development project |
| ||
| 2B08060, research and development project |
|