Plasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling.

DHONUKSHE, Pankaj, Fang HUANG, Carlos S. GALAN-AMPUDIA, Ari Pekka MÄHÖNEN, Jurgen KLEINE-VEHN, Jian XU, Ab QIUNT, Kalika PRASAD, Jiří FRIML, Ben SCHERES and Offringa REMKO. Plasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling. Development. Cambridge: Company of Biologists, 2010, vol. 137, No 19, p. 3145-3255, 11 pp. ISSN 0950-1991.

Basic information

Original name

Plasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling.

Name in Czech

AGC3 kinázy na plazmatické membráně fosforylují PIN auxinové přenašeče a tím regulují apikální recyklování PIN protein

Authors

DHONUKSHE, Pankaj, Fang HUANG, Carlos S. GALAN-AMPUDIA, Ari Pekka MÄHÖNEN, Jurgen KLEINE-VEHN, Jian XU, Ab QIUNT, Kalika PRASAD, Jiří FRIML, Ben SCHERES and Offringa REMKO

Edition

Development, Cambridge, Company of Biologists, 2010, 0950-1991

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.898

Organization unit

Central European Institute of Technology

UT WoS

000281608200011

Keywords (in Czech)

Polární transport auxinu; PIN auxinové přenašeče; PID; WAG1; WAG2; buněčná polarita; transcytóza

Keywords in English

Polar auxin transport; PIN efflux carrier; PID; WAG1; WAG2; Cell polarity Transcytosis

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Abstract

V originále

Polar membrane cargo delivery is crucial for establishing cell polarity and for directional transport processes. In plants, polar trafficking mediates the dynamic asymmetric distribution of PIN FORMED (PIN) carriers, which drive polar cell-to-cell transport of the hormone auxin, thereby generating auxin maxima and minima that control development. The Arabidopsis PINOID (PID)protein kinase instructs apical PIN localization by phosphorylating PINs. Here, we identified the PID homologs WAG1 and WAG2 as new PIN polarity regulators. We show that the AGC3 kinases PID, WAG1 and WAG2, and not other plant AGC kinases, instruct recruitment of PINs into the apical recycling pathway by phosphorylating the middle serine in three conserved TPRXS(N/S) motifs within the PIN central hydrophilic loop. Our results put forward a model by which apolarly localized PID, WAG1 and WAG2 phosphorylate PINs at the plasma membrane after default non-polar PIN secretion, and trigger endocytosis-dependent apical PIN recycling. This phosphorylation-triggered apical PIN recycling competes with ARF-GEF GNOM-dependent basal recycling to promote apical PIN localization. In planta, expression domains of PID, WAG1 and WAG2 correlate with apical localization of PINs in those cell types, indicating the importance of these kinases for apical PIN localization. Our data show that by directing polar PIN localization and PIN-mediated polar auxin transport, the three AGC3 kinases redundantly regulate cotyledon development, root meristem size and gravitropic response, indicating their involvement in both programmed and adaptive plant development.

In Czech

Polární depozice proteinů na plazmatickou membránu je důležité pro buněčnou polaritu. V tomto článku je ukázáno, že PINOID a podobné kinázy regulují buněčnou polaritu. Aktivita těchto kináz fosforyluje PIN proteiny a takto způsobuje apikální lokalizaci PIN proteinů a takto reguluje vývojové procesy včetně vývoje embryonálních listů, velikost kořenového meristému a gravitropní růst.