Preparation of protein conjugates with highly hydrophobic
molecules and their use in  heterogeneous immunoassay

D 2008

Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay

FRNKOVÁ, Kateřina, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK

Basic information

Original name

Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay

Authors

FRNKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Miloslav SUCHÁNEK (203 Czech Republic)

Edition

Brno, 5th International Interdisciplinary Meeting on Bioanalysis CECE2008, p. 60-61, 2 pp. 2008

Publisher

Institute of Analytical Chemisty AS CR

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/08:00052275

Organization unit

Faculty of Science

ISBN

978-80-254-3194-8

Keywords in English

bioconjugates immunization ELISA

Abstract

V originále

Chromatogpraphic methods coupled to a mass spectroscopy are widely used in analysi of PAHs and their metabolites, as those methods provide high sensitivity and selectivity, combined with excellent robustness. Immunoanalytical methods, such as ELISA, offer interesting possibility to detect PAHs at low levels with low expanses. Moreover, development of an antibody with specifity to polyaromatic hydrocarbons offers a possibility to detect DNA-PAH adducts. Preparation and characterization of immunoconjugates of polyaromatic hydrocarbon model compound (pyrenebutyric acid) with proteins (carrier proteins and enzymes) is described (shown as scheme on this page). PAHs compounds as highly lipophilic molecules can not be conjugated to a protein structure in a single step procedure, however, step-by-step changing of conjugation and purification steps needs to be employed in this way. Use of prepared conjugates in construction of direct and indirect ELISA method is shown, too.

Links

2B06056, research and development project

Name: Diagnostika poškození DNA polyaromatickými sloučeninami použitím nanotechnologických a bioanalytických metod pro včasnou detekci karcinomu.

Investor: Ministry of Education, Youth and Sports of the CR, Nanotechnological and bioanalytical detection of the DNA damage

Citovat

FRNKOVÁ, Kateřina, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK. Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay. In 5th International Interdisciplinary Meeting on Bioanalysis CECE2008. Brno: Institute of Analytical Chemisty AS CR, 2008, p. 60-61. ISBN 978-80-254-3194-8.

@inproceedings{937681,
   author = {Frnková, Kateřina and Přibyl, Jan and Skládal, Petr and Suchánek, Miloslav},
   address = {Brno},
   booktitle = {5th International Interdisciplinary Meeting on Bioanalysis CECE2008},
   keywords = {bioconjugates immunization ELISA},
   language = {eng},
   location = {Brno},
   isbn = {978-80-254-3194-8},
   pages = {60-61},
   publisher = {Institute of Analytical Chemisty AS CR},
   title = {Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay},
   year = {2008}
}

TY  - JOUR
ID  - 937681
AU  - Frnková, Kateřina - Přibyl, Jan - Skládal, Petr - Suchánek, Miloslav
PY  - 2008
TI  - Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay
PB  - Institute of Analytical Chemisty AS CR
CY  - Brno
SN  - 9788025431948
KW  - bioconjugates immunization ELISA
N2  - Chromatogpraphic methods coupled to a mass spectroscopy are widely used in analysi of PAHs and their metabolites, as those methods provide high sensitivity and selectivity, combined with excellent robustness. Immunoanalytical methods, such as ELISA, offer interesting possibility to detect PAHs at low levels with low expanses. Moreover, development of an antibody with specifity to polyaromatic hydrocarbons offers a possibility to detect DNA-PAH adducts. Preparation and characterization of immunoconjugates of polyaromatic hydrocarbon model compound (pyrenebutyric acid) with proteins (carrier proteins and enzymes) is described (shown as scheme on this page). PAHs compounds as highly lipophilic molecules can not be conjugated to a protein structure in a single step procedure, however, step-by-step changing of conjugation and purification steps needs to be employed in this way. Use of prepared conjugates in construction of direct and indirect ELISA method is shown, too.
ER  -

FRNKOVÁ, Kateřina, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK. Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay. In \textit{5th International Interdisciplinary Meeting on Bioanalysis CECE2008}. Brno: Institute of Analytical Chemisty AS CR, 2008, p.~60-61. ISBN~978-80-254-3194-8.

Detailed Information on Publication Record