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@inproceedings{937681, author = {Frnková, Kateřina and Přibyl, Jan and Skládal, Petr and Suchánek, Miloslav}, address = {Brno}, booktitle = {5th International Interdisciplinary Meeting on Bioanalysis CECE2008}, keywords = {bioconjugates immunization ELISA}, language = {eng}, location = {Brno}, isbn = {978-80-254-3194-8}, pages = {60-61}, publisher = {Institute of Analytical Chemisty AS CR}, title = {Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay}, year = {2008} }
TY - JOUR ID - 937681 AU - Frnková, Kateřina - Přibyl, Jan - Skládal, Petr - Suchánek, Miloslav PY - 2008 TI - Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay PB - Institute of Analytical Chemisty AS CR CY - Brno SN - 9788025431948 KW - bioconjugates immunization ELISA N2 - Chromatogpraphic methods coupled to a mass spectroscopy are widely used in analysi of PAHs and their metabolites, as those methods provide high sensitivity and selectivity, combined with excellent robustness. Immunoanalytical methods, such as ELISA, offer interesting possibility to detect PAHs at low levels with low expanses. Moreover, development of an antibody with specifity to polyaromatic hydrocarbons offers a possibility to detect DNA-PAH adducts. Preparation and characterization of immunoconjugates of polyaromatic hydrocarbon model compound (pyrenebutyric acid) with proteins (carrier proteins and enzymes) is described (shown as scheme on this page). PAHs compounds as highly lipophilic molecules can not be conjugated to a protein structure in a single step procedure, however, step-by-step changing of conjugation and purification steps needs to be employed in this way. Use of prepared conjugates in construction of direct and indirect ELISA method is shown, too. ER -
FRNKOVÁ, Kateřina, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK. Preparation of protein conjugates with highly hydrophobic molecules and their use in heterogeneous immunoassay. In \textit{5th International Interdisciplinary Meeting on Bioanalysis CECE2008}. Brno: Institute of Analytical Chemisty AS CR, 2008, p.~60-61. ISBN~978-80-254-3194-8.
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