Chemically functionalized AFM tips: easy way to resolution
enhancement in single biomolecule visualization.

D 2008

Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.

KŘÍŽ, Martin, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK

Basic information

Original name

Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.

Authors

KŘÍŽ, Martin (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Miloslav SUCHÁNEK (203 Czech Republic)

Edition

Brno, 5th international interdisciplinary meeting on bioanalysis CECE 2008, p. 65-66, 2 pp. 2008

Publisher

Institute of Analytical Chemisty AS CR

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/08:00052276

Organization unit

Faculty of Science

ISBN

978-80-254-3194-8

Keywords in English

AFM microscope cantilever tip silanization hydrophobicity

Abstract

V originále

Atomic Force Microscopy (AFM) technique allows visualization of structures in a sub-nanometer scale, i.e. imaging of individual biomolecules. In principle AFM scanning probe, presented by ultra-sharp tip, is in close contact with surface and relief is visualized as 3D map (after conversion of a measured quantity, e.g. tip bending). Resolution of an AFM microscope is essentially affected by the tip curvature (sharpness), however other experimental conditions would affect this parameter, too. Only weak and medium forces are usually employed in the interaction between tip and surface in the relief visualization. Surfaces visualized in a non-controlled atmosphere are naturally coated with a very thin (1-5 nm) layer of adsorbed water. In some cases capillary forces originating from this layer can strongly affect visualization process, as those forces are much stronger comparing to other intermolecular forces (van derWaals, hydrogen, ionic forces, etc.). Formation of capillary forces can be effectively suppressed by chemical modification of tip (mostly by hydrophobization of the tip) surface. Various methods for tip surface modification are presented. Procedure always starts with the surface activation by a silanization. Subsequent coupling of either hydrophilic or hydrophobic molecules determines surface properties in this way. Impact of the tip surface modification on resolution of images of individual biomolecules (proteins, nucleic acids) are shown as examples (on the right: IgG molecule-hydrophilic tip, DNA-lipophilic tip).

Links

2B06056, research and development project

Name: Diagnostika poškození DNA polyaromatickými sloučeninami použitím nanotechnologických a bioanalytických metod pro včasnou detekci karcinomu.

Investor: Ministry of Education, Youth and Sports of the CR, Nanotechnological and bioanalytical detection of the DNA damage

Citovat

KŘÍŽ, Martin, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK. Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization. In 5th international interdisciplinary meeting on bioanalysis CECE 2008. Brno: Institute of Analytical Chemisty AS CR, 2008, p. 65-66. ISBN 978-80-254-3194-8.

@inproceedings{937682,
   author = {Kříž, Martin and Přibyl, Jan and Skládal, Petr and Suchánek, Miloslav},
   address = {Brno},
   booktitle = {5th international interdisciplinary meeting on bioanalysis CECE 2008},
   keywords = {AFM microscope cantilever tip silanization hydrophobicity},
   language = {eng},
   location = {Brno},
   isbn = {978-80-254-3194-8},
   pages = {65-66},
   publisher = {Institute of Analytical Chemisty AS CR},
   title = {Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.},
   year = {2008}
}

TY  - JOUR
ID  - 937682
AU  - Kříž, Martin - Přibyl, Jan - Skládal, Petr - Suchánek, Miloslav
PY  - 2008
TI  - Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.
PB  - Institute of Analytical Chemisty AS CR
CY  - Brno
SN  - 9788025431948
KW  - AFM microscope cantilever tip silanization hydrophobicity
N2  - Atomic Force Microscopy (AFM) technique allows visualization of structures in a sub-nanometer scale, i.e. imaging of individual biomolecules. In principle AFM scanning probe, presented by ultra-sharp tip, is in close contact with surface and relief is visualized as 3D map (after conversion of a measured quantity, e.g. tip bending). Resolution of an AFM microscope is essentially affected by the tip curvature (sharpness), however other experimental conditions would affect this parameter, too. Only weak and medium forces are usually employed in the interaction between tip and surface in the relief visualization. Surfaces visualized in a non-controlled atmosphere are naturally coated with a very thin (1-5 nm) layer of adsorbed water. In some cases capillary forces originating from this layer can strongly affect visualization process, as those forces are much stronger comparing to other intermolecular forces (van derWaals, hydrogen, ionic forces, etc.). Formation of capillary forces can be effectively suppressed by chemical modification of tip (mostly by hydrophobization of the tip) surface. Various methods for tip surface modification are presented. Procedure always starts with the surface activation by a silanization. Subsequent coupling of either hydrophilic or hydrophobic molecules determines surface properties in this way. Impact of the tip surface modification on resolution of images of individual biomolecules (proteins, nucleic acids) are shown as examples (on the right: IgG molecule-hydrophilic tip, DNA-lipophilic tip).
ER  -

KŘÍŽ, Martin, Jan PŘIBYL, Petr SKLÁDAL and Miloslav SUCHÁNEK. Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization. In \textit{5th international interdisciplinary meeting on bioanalysis CECE 2008}. Brno: Institute of Analytical Chemisty AS CR, 2008, p.~65-66. ISBN~978-80-254-3194-8.

Detailed Information on Publication Record