a 2011

APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

CASTILLO, Gabriela, Libuše TRNKOVÁ, Marcos Vinicius FOGUEL and Tibor HIANIK

Basic information

Original name

APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

Authors

CASTILLO, Gabriela (218 Ecuador, belonging to the institution), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Marcos Vinicius FOGUEL (76 Brazil) and Tibor HIANIK (703 Slovakia, guarantor)

Edition

XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS, 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10403 Physical chemistry

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/11:00052423

Organization unit

Faculty of Science

ISBN

978-83-62139-23-1

Keywords in English

DNA;RNA;aptamers;thrombin;gold cd;electrochemical impedance

Tags

Změněno: 13/4/2012 12:35, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Biosensors based on DNA or RNA aptamers, represent a very efficient platform for protein recognition1. We developed a novel label-free aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein complex formation using gold CD-trodes (GCDT). GCDT are electrodes fabricated from recordable compact discs that contain a fine gold layer on their surface. Low-cost GCDTs present good versatility and acceptable reproducibility. The thrombin biosensor is constructed by self-assembling a thiol-modified aptamer onto a GCDT surface, followed by recognition of the protein via monitoring the interfacial electron transfer resistance in the presence of an [Fe(CN)6]3-/4- redox couple2,3. The aptasensor was also characterized by cyclic voltammetry and the individual binding steps were analysed by scanning electron microscopy.

Links

LC06035, research and development project
Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)