APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY

CASTILLO, Gabriela, Libuše TRNKOVÁ, Marcos Vinicius FOGUEL and Tibor HIANIK. APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY. In XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS. 2011. ISBN 978-83-62139-23-1.

Basic information

• Original name: APTAMER BASED CD-SENSORS FOR THROMBIN RECOGNITION BY MEANS OF ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
• Authors: CASTILLO, Gabriela (218 Ecuador, belonging to the institution), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Marcos Vinicius FOGUEL (76 Brazil) and Tibor HIANIK (703 Slovakia, guarantor).
• Edition: XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10403 Physical chemistry
• Country of publisher: Poland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/11:00052423
• Organization unit: Faculty of Science
• ISBN: 978-83-62139-23-1
• Keywords in English: DNA;RNA;aptamers;thrombin;gold cd;electrochemical impedance
• Tags: AKR

Abstract

Biosensors based on DNA or RNA aptamers, represent a very efficient platform for protein recognition1. We developed a novel label-free aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein complex formation using gold CD-trodes (GCDT). GCDT are electrodes fabricated from recordable compact discs that contain a fine gold layer on their surface. Low-cost GCDTs present good versatility and acceptable reproducibility. The thrombin biosensor is constructed by self-assembling a thiol-modified aptamer onto a GCDT surface, followed by recognition of the protein via monitoring the interfacial electron transfer resistance in the presence of an [Fe(CN)6]3-/4- redox couple2,3. The aptasensor was also characterized by cyclic voltammetry and the individual binding steps were analysed by scanning electron microscopy.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)