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@proceedings{941090,
author = {Kryštofová, Olga and Vysloužil, Libor and Eliáš, Marek and Huska, Dalibor and Sochor, Jiří and Zítka, Ondřej and Adam, Vojtěch and Kudrle, Vít and Hubalek, Jaromir and Trnková, Libuše and Beklova, Miroslava and Kizek, René},
booktitle = {XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS},
keywords = {nanoparticles;magnetic nanoparticles;PECVD;DPV;Brdicka reaction},
language = {eng},
isbn = {978-83-62139-23-1},
title = {INFLUENCE OF CARBON AND PARAMAGNETIC PARTICLES ON TOBACCO BY-2 CELL CULTURE},
year = {2011}
}
TY - CONF
ID - 941090
AU - Kryštofová, Olga - Vysloužil, Libor - Eliáš, Marek - Huska, Dalibor - Sochor, Jiří - Zítka, Ondřej - Adam, Vojtěch - Kudrle, Vít - Hubalek, Jaromir - Trnková, Libuše - Beklova, Miroslava - Kizek, René
PY - 2011
TI - INFLUENCE OF CARBON AND PARAMAGNETIC PARTICLES ON TOBACCO BY-2 CELL CULTURE
SN - 9788362139231
KW - nanoparticles;magnetic nanoparticles;PECVD;DPV;Brdicka reaction
N2 - One of nanotechnological goals is using the nanoparticles (carbon,magnetic) for targeted drug delivery or advanced in vivo and in vitro analytical detection of specific biomolecules and/or imaging diagnostics. However, careful investigation of possible adverse effects must be carried out. Among principal methods of the nanoparticle synthesis there are conferred co-precipitation, thermal deposition and laser pyrolysis. In this study, we have used low pressure microwave PECVD process for preparation of iron based nanoparticles and coated by several methods. The synthesised nanopowders (0,1,10 and 100mikrog/ml) were then tested for bioactivity and biotoxicity on plant BY-2 tobacco cells. For this purpose, we have used determination cell viability, differential pulse voltammetry Brdicka reaction (total content of thiols) and liquid chromatography with electrochemical detection (glutathiones) and spectrometry (antioxidant activity). We have found the great differences between coated and un-coated particles, because the un-coated have had much greater toxicity than –COOH coated. Moreover, the un-coated particles caused enhancement of level of thiols and antioxidant activity, which relates to their adverse effects on the cells.
ER -
KRYŠTOFOVÁ, Olga, Libor VYSLOUŽIL, Marek ELIÁŠ, Dalibor HUSKA, Jiří SOCHOR, Ondřej ZÍTKA, Vojtěch ADAM, Vít KUDRLE, Jaromir HUBALEK, Libuše TRNKOVÁ, Miroslava BEKLOVA a René KIZEK. INFLUENCE OF CARBON AND PARAMAGNETIC PARTICLES ON TOBACCO BY-2 CELL CULTURE. In \textit{XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS}. 2011. ISBN~978-83-62139-23-1.
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