REISS, S., Ilka REBHAN, P. BACKES, I. ROMERO-BREY, Holger ERFLE, Petr MATULA, Lars KADERALI, Marion POENISCH, H. BLANKENBURG, M.S. HIET, T. LONGERICH, S. DIEHL, F. RAMIREZ, T. BALLA, Karl ROHR, A. KAUL, S. BÜHLER, Rainer PEPPERKOK, T. LENGAUER, M. ALBRECHT, Roland EILS, P. SCHIRMACHER, V. LOHMANN and Ralf BARTENSCHLAGER. Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. Cell Host & Microbe, 2011, vol. 9, No 1, p. 32-45. ISSN 1931-3128. doi:10.1016/j.chom.2010.12.002.
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Basic information
Original name Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.
Authors REISS, S. (276 Germany), Ilka REBHAN (276 Germany), P. BACKES (276 Germany), I. ROMERO-BREY (276 Germany), Holger ERFLE (276 Germany), Petr MATULA (203 Czech Republic, guarantor, belonging to the institution), Lars KADERALI (276 Germany), Marion POENISCH (276 Germany), H. BLANKENBURG (276 Germany), M.S. HIET (276 Germany), T. LONGERICH (276 Germany), S. DIEHL (276 Germany), F. RAMIREZ (276 Germany), T. BALLA (276 Germany), Karl ROHR (276 Germany), A. KAUL (276 Germany), S. BÜHLER (276 Germany), Rainer PEPPERKOK (276 Germany), T. LENGAUER (276 Germany), M. ALBRECHT (276 Germany), Roland EILS (276 Germany), P. SCHIRMACHER (276 Germany), V. LOHMANN (276 Germany) and Ralf BARTENSCHLAGER (276 Germany).
Edition Cell Host & Microbe, 2011, 1931-3128.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 13.500
RIV identification code RIV/00216224:14330/11:00052648
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1016/j.chom.2010.12.002
UT WoS 000287344500006
Keywords in English RNA REPLICATION; PHOSPHATIDYLINOSITOL 4-KINASES; CELLULAR COFACTORS; CORE PROTEIN; HOST FACTORS; HUH-7 CELLS; INFECTION; COMPLEX; IDENTIFICATION; SCREEN
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Petr Matula, Ph.D., učo 3019. Changed: 4/6/2012 23:25.
Abstract
Hepatitis C virus (HCV) is a major causative agent of chronic liver disease in humans. To gain insight into host factor requirements for HCV replication, we performed a siRNA screen of the human kinome and identified 13 different kinases, including phosphatidylinositol-4 kinase III alpha (PI4KIIIalfa), as being required for HCV replication. Consistent with elevated levels of the PI4KIIIalfa product phosphatidylinositol-4-phosphate (PI4P) detected in HCV-infected cultured hepatocytes and liver tissue from chronic hepatitis C patients, the enzymatic activity of PI4KIIIalfa was critical for HCV replication. Viral nonstructural protein 5A (NS5A) was found to interact with PI4KIIIalfa and stimulate its kinase activity. The absence of PI4KIIIalfa activity induced a dramatic change in the ultrastructural morphology of the membranous HCV replication complex. Our analysis suggests that the direct activation of a lipid kinase by HCV NS5A contributes critically to the integrity of the membranous viral replication complex.
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MSM0021622419, plan (intention)Name: Vysoce paralelní a distribuované výpočetní systémy
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
2B06052, research and development projectName: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)
Investor: Ministry of Education, Youth and Sports of the CR, Health and quality of life
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