| REISS, S., Ilka REBHAN, P. BACKES, I. ROMERO-BREY, Holger ERFLE, Petr MATULA, Lars KADERALI, Marion POENISCH, H. BLANKENBURG, M.S. HIET, T. LONGERICH, S. DIEHL, F. RAMIREZ, T. BALLA, Karl ROHR, A. KAUL, S. BÜHLER, Rainer PEPPERKOK, T. LENGAUER, M. ALBRECHT, Roland EILS, P. SCHIRMACHER, V. LOHMANN and Ralf BARTENSCHLAGER. Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. Cell Host & Microbe. vol. 9, No 1, p. 32-45. ISSN 1931-3128. doi:10.1016/j.chom.2010.12.002. 2011. |
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@article{945118,
author = {Reiss, S. and Rebhan, Ilka and Backes, P. and RomeroandBrey, I. and Erfle, Holger and Matula, Petr and Kaderali, Lars and Poenisch, Marion and Blankenburg, H. and Hiet, M.S. and Longerich, T. and Diehl, S. and Ramirez, F. and Balla, T. and Rohr, Karl and Kaul, A. and Bühler, S. and Pepperkok, Rainer and Lengauer, T. and Albrecht, M. and Eils, Roland and Schirmacher, P. and Lohmann, V. and Bartenschlager, Ralf},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.chom.2010.12.002},
keywords = {RNA REPLICATION; PHOSPHATIDYLINOSITOL 4-KINASES; CELLULAR COFACTORS; CORE PROTEIN; HOST FACTORS; HUH-7 CELLS; INFECTION; COMPLEX; IDENTIFICATION; SCREEN},
language = {eng},
issn = {1931-3128},
journal = {Cell Host & Microbe},
title = {Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.},
volume = {9},
year = {2011}
}
TY - JOUR
ID - 945118
AU - Reiss, S. - Rebhan, Ilka - Backes, P. - Romero-Brey, I. - Erfle, Holger - Matula, Petr - Kaderali, Lars - Poenisch, Marion - Blankenburg, H. - Hiet, M.S. - Longerich, T. - Diehl, S. - Ramirez, F. - Balla, T. - Rohr, Karl - Kaul, A. - Bühler, S. - Pepperkok, Rainer - Lengauer, T. - Albrecht, M. - Eils, Roland - Schirmacher, P. - Lohmann, V. - Bartenschlager, Ralf
PY - 2011
TI - Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.
JF - Cell Host & Microbe
VL - 9
IS - 1
SP - 32-45
EP - 32-45
SN - 19313128
KW - RNA REPLICATION
KW - PHOSPHATIDYLINOSITOL 4-KINASES
KW - CELLULAR COFACTORS
KW - CORE PROTEIN
KW - HOST FACTORS
KW - HUH-7 CELLS
KW - INFECTION
KW - COMPLEX
KW - IDENTIFICATION
KW - SCREEN
N2 - Hepatitis C virus (HCV) is a major causative agent of chronic liver disease in humans. To gain insight into host factor requirements for HCV replication, we performed a siRNA screen of the human kinome and identified 13 different kinases, including phosphatidylinositol-4 kinase III alpha (PI4KIIIalfa), as being required for HCV replication. Consistent with elevated levels of the PI4KIIIalfa product phosphatidylinositol-4-phosphate (PI4P) detected in HCV-infected cultured hepatocytes and liver tissue from chronic hepatitis C patients, the enzymatic activity of PI4KIIIalfa was critical for HCV replication. Viral nonstructural protein 5A (NS5A) was found to interact with PI4KIIIalfa and stimulate its kinase activity. The absence of PI4KIIIalfa activity induced a dramatic change in the ultrastructural morphology of the membranous HCV replication complex. Our analysis suggests that the direct activation of a lipid kinase by HCV NS5A contributes critically to the integrity of the membranous viral replication complex.
ER -
REISS, S., Ilka REBHAN, P. BACKES, I. ROMERO-BREY, Holger ERFLE, Petr MATULA, Lars KADERALI, Marion POENISCH, H. BLANKENBURG, M.S. HIET, T. LONGERICH, S. DIEHL, F. RAMIREZ, T. BALLA, Karl ROHR, A. KAUL, S. BÜHLER, Rainer PEPPERKOK, T. LENGAUER, M. ALBRECHT, Roland EILS, P. SCHIRMACHER, V. LOHMANN and Ralf BARTENSCHLAGER. Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment. \textit{Cell Host \&{} Microbe}. vol.~9, No~1, p.~32-45. ISSN~1931-3128. doi:10.1016/j.chom.2010.12.002. 2011.
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