Detailed Information on Publication Record
2011
Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities
ŠEBESTA, Marek, Peter BURKOVICS, Lajos HARACSKA and Lumír KREJČÍBasic information
Original name
Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities
Authors
ŠEBESTA, Marek (703 Slovakia, belonging to the institution), Peter BURKOVICS (348 Hungary), Lajos HARACSKA (348 Hungary) and Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)
Edition
DNA Repair, 2011, 1568-7864
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.135
RIV identification code
RIV/00216224:14110/11:00049878
Organization unit
Faculty of Medicine
UT WoS
000292440800002
Keywords in English
DNA repair; Recombination; DNA synthesis; Replication; Mph1 Srs2
Tags
International impact
Změněno: 22/3/2012 15:13, Mgr. Michal Petr
Abstract
V originále
The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. While some of the aspects of the initial steps of HR are understood, the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. Therefore, we have reconstituted D-loop-based in vitro recombinationassociated DNA repair synthesis assay and tested the efficacy of polymerases Pol and Pol to extend invaded primer, and the ability of three helicases (Mph1, Srs2 and Sgs1) to displace this extended primer. Both Pol and Pol extended up to 50% of the D-loop substrate, but differed in product length and dependency on proliferating cell nuclear antigen (PCNA). Mph1, but not Srs2 or Sgs1, displaced the extended primer very efficiently, supporting putative role ofMph1in promoting the synthesis-dependent strand-annealing pathway. The experimental system described here can be employed to increase our understanding of HR events following D-loop formation, as well as the regulatory mechanisms involved.
Links
GA301/09/1917, research and development project |
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GD203/09/H046, research and development project |
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LC06030, research and development project |
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ME10048, research and development project |
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