An accumulation of heavy metals in different bacterial strains in soil has been observed and could be used in the process of remediation of soils contaminated by heavy-metal-pollutants. In this study, Streptomyces, well known for their capacity to accumulate heavy metals from the environment, were selected. The ability to accumulate heavy metals in biomass was determined for three strains both collection strain (CCM3243) and two strains isolated from soil exposed to heavy traffic emissions in the centre of Brno (ON3 and M).These strains were cultivated in the presence of different concentrations of zinc(II) (0, 50 and 100 uM) and copper(II) ions (0, 100, 200 and 500 uM). Limit of detection estimated as 3 S/N was 1 nM for zinc(II) ions and limit of quantification as 10 S/N was 5 nM. The accumulation data obtained correlate very well with the results of biological experiments, especially ON3 strain with the highest resistance also has the ability to accumulate highest concentration of metal ions in the cell wall.