| ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK a Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, roč. 56, č. 1, s. 72-76. ISSN 0015-5632. doi:10.1007/s12223-011-0004-6. 2011. |
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@article{947101,
author = {Illichmanová, Adéla and Janů, Miroslava and Sedláček, Vojtěch and Kučera, Igor},
article_location = {Dordrecht, The Netherlands},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s12223-011-0004-6},
keywords = {nitrate reductase; artificial electron donor; nitrate transport; inhibition},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen},
url = {http://www.springerlink.com/content/u876462762641134/},
volume = {56},
year = {2011}
}
TY - JOUR
ID - 947101
AU - Illichmanová, Adéla - Janů, Miroslava - Sedláček, Vojtěch - Kučera, Igor
PY - 2011
TI - Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
JF - Folia Microbiologica
VL - 56
IS - 1
SP - 72-76
EP - 72-76
PB - Springer
SN - 00155632
KW - nitrate reductase
KW - artificial electron donor
KW - nitrate transport
KW - inhibition
UR - http://www.springerlink.com/content/u876462762641134/
N2 - A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
ER -
ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK a Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. \textit{Folia Microbiologica}. Dordrecht, The Netherlands: Springer, roč.~56, č.~1, s.~72-76. ISSN~0015-5632. doi:10.1007/s12223-011-0004-6. 2011.
|
