ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, 2011, vol. 56, No 1, p. 72-76. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6. |
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@article{947101, author = {Illichmanová, Adéla and Janů, Miroslava and Sedláček, Vojtěch and Kučera, Igor}, article_location = {Dordrecht, The Netherlands}, article_number = {1}, doi = {http://dx.doi.org/10.1007/s12223-011-0004-6}, keywords = {nitrate reductase; artificial electron donor; nitrate transport; inhibition}, language = {eng}, issn = {0015-5632}, journal = {Folia Microbiologica}, title = {Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen}, url = {http://www.springerlink.com/content/u876462762641134/}, volume = {56}, year = {2011} }
TY - JOUR ID - 947101 AU - Illichmanová, Adéla - Janů, Miroslava - Sedláček, Vojtěch - Kučera, Igor PY - 2011 TI - Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen JF - Folia Microbiologica VL - 56 IS - 1 SP - 72-76 EP - 72-76 PB - Springer SN - 00155632 KW - nitrate reductase KW - artificial electron donor KW - nitrate transport KW - inhibition UR - http://www.springerlink.com/content/u876462762641134/ N2 - A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter. ER -
ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. \textit{Folia Microbiologica}. Dordrecht, The Netherlands: Springer, 2011, vol.~56, No~1, p.~72-76. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6.
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