ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen. Folia Microbiologica. Dordrecht, The Netherlands: Springer, 2011, vol. 56, No 1, p. 72-76. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-011-0004-6.
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Basic information
Original name Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
Name in Czech Stanovení nitrátreduktasy v celých buňkách: vedlejší účinky při použití benzylviologenu
Authors ILLICHMANOVÁ, Adéla (203 Czech Republic, belonging to the institution), Miroslava JANŮ (203 Czech Republic, belonging to the institution), Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution) and Igor KUČERA (203 Czech Republic, guarantor, belonging to the institution).
Edition Folia Microbiologica, Dordrecht, The Netherlands, Springer, 2011, 0015-5632.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.677
RIV identification code RIV/00216224:14310/11:00052814
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s12223-011-0004-6
UT WoS 000290451500012
Keywords (in Czech) nitrátreduktasa; umělý elektronový donor; transport dusičnanu; inhibice
Keywords in English nitrate reductase; artificial electron donor; nitrate transport; inhibition
Tags AKR, rivok
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 12/4/2012 12:31.
Abstract
A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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