Heterogeneity in the kinetics of nuclear proteins and
trajectories of substructures associated with heterochromatin

J 2011

Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ et. al.

Basic information

Original name

Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

Authors

STIXOVÁ, Lenka (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Pavel MATULA (203 Czech Republic, guarantor, belonging to the institution), Ondřej DANĚK (203 Czech Republic, belonging to the institution), Soňa LEGARTOVÁ (703 Slovakia) and Stanislav KOZUBEK (203 Czech Republic)

Edition

Epigenetics & Chromatin, 2011, 1756-8935

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.462

RIV identification code

RIV/00216224:14330/11:00052815

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1186/1756-8935-4-5

UT WoS

000288995300001

Keywords in English

protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin

Abstract

V originále

We show that protein dynamics are influenced by many factors and events, including nuclear pattern and transcription activity. A slower recovery after photobleaching was found when proteins, such as HP1b, BMI1, TRF1, and others accumulated at specific foci. In identical cells, proteins that were evenly dispersed throughout the nucleoplasm recovered more rapidly. Distinct trajectories for HP1b, BMI1, and TRF1 were observed after hyperacetylation or suppression of transcription. The relationship between protein trajectory and transcription level was confirmed for telomeric protein TRF1, but not for HP1b or BMI1 proteins. Moreover, heterogeneity of foci movement was especially observed when we made distinctions between centrally and peripherally positioned foci. Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins.

Links

LC535, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Investor: Ministry of Education, Youth and Sports of the CR, Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Citovat

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ and Stanislav KOZUBEK. Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin. Epigenetics & Chromatin. 2011, vol. 4, No 5, p. 1-17. ISSN 1756-8935. Available from: https://dx.doi.org/10.1186/1756-8935-4-5.

@article{947102,
   author = {Stixová, Lenka and Bártová, Eva and Matula, Pavel and Daněk, Ondřej and Legartová, Soňa and Kozubek, Stanislav},
   article_number = {5},
   doi = {http://dx.doi.org/10.1186/1756-8935-4-5},
   keywords = {protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin},
   language = {eng},
   issn = {1756-8935},
   journal = {Epigenetics & Chromatin},
   title = {Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin},
   url = {http://www.epigeneticsandchromatin.com/content/4/1/5},
   volume = {4},
   year = {2011}
}

TY  - JOUR
ID  - 947102
AU  - Stixová, Lenka - Bártová, Eva - Matula, Pavel - Daněk, Ondřej - Legartová, Soňa - Kozubek, Stanislav
PY  - 2011
TI  - Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin
JF  - Epigenetics & Chromatin
VL  - 4
IS  - 5
SP  - 1-17
EP  - 1-17
SN  - 17568935
KW  - protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin
UR  - http://www.epigeneticsandchromatin.com/content/4/1/5
L2  - http://www.epigeneticsandchromatin.com/content/4/1/5
N2  - We show that protein dynamics are influenced by many factors and events, including nuclear pattern and transcription activity. A slower recovery after photobleaching was found when proteins, such as HP1b, BMI1, TRF1, and others accumulated at specific foci. In identical cells, proteins that were evenly dispersed throughout the nucleoplasm recovered more rapidly. Distinct trajectories for HP1b, BMI1, and TRF1 were observed after hyperacetylation or suppression of transcription. The relationship between protein trajectory and transcription level was confirmed for telomeric protein TRF1, but not for HP1b or BMI1 proteins. Moreover, heterogeneity of foci movement was especially observed when we made distinctions between centrally and peripherally positioned foci. Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins.
ER  -

STIXOVÁ, Lenka, Eva BÁRTOVÁ, Pavel MATULA, Ondřej DANĚK, Soňa LEGARTOVÁ and Stanislav KOZUBEK. Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin. \textit{Epigenetics \&{} Chromatin}. 2011, vol.~4, No~5, p.~1-17. ISSN~1756-8935. Available from: https://dx.doi.org/10.1186/1756-8935-4-5.

Detailed Information on Publication Record