Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

PRUDNIKOVA, Tanyana, Radka CHALOUPKOVÁ, Yukari SATO, Yuji NAGATA, O. DEGTJARIK, Michal KUTÝ, P. REZACOVA, Jiří DAMBORSKÝ and Smatanova I. KUTA-SMATANOVÁ. Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94. Crystal Growth & Design. 2011, vol. 11, No 2, p. 516-519. ISSN 1528-7483. Available from: https://dx.doi.org/10.1021/cg1013363.

Basic information

• Original name: Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
• Name in Czech: Vývoj krystalizace protokolu pro DbeA1 Variant nových dehalogenázy Haloalkane z Bradyrhizobium elkani USDA94
• Authors: PRUDNIKOVA, Tanyana (112 Belarus), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Yukari SATO (392 Japan), Yuji NAGATA (392 Japan), O. DEGTJARIK (112 Belarus), Michal KUTÝ (203 Czech Republic), P. REZACOVA (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Smatanova I. KUTA-SMATANOVÁ (203 Czech Republic).
• Edition: Crystal Growth & Design, 2011, 1528-7483.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.720
• RIV identification code: RIV/00216224:14310/11:00049438
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1021/cg1013363
• UT WoS: 000286714200021
• Keywords in English: Crystallization; X-ray;Haloalkane dehalogenases
• Tags: AKR, rivok

Abstract

The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment