PRUDNIKOVA, Tanyana, Radka CHALOUPKOVÁ, Yukari SATO, Yuji NAGATA, O. DEGTJARIK, Michal KUTÝ, P. REZACOVA, Jiří DAMBORSKÝ and Smatanova I. KUTA-SMATANOVÁ. Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94. Crystal Growth & Design. 2011, vol. 11, No 2, p. 516-519. ISSN 1528-7483. Available from: https://dx.doi.org/10.1021/cg1013363. |
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@article{947493, author = {Prudnikova, Tanyana and Chaloupková, Radka and Sato, Yukari and Nagata, Yuji and Degtjarik, O. and Kutý, Michal and Rezacova, P. and Damborský, Jiří and KutaandSmatanová, Smatanova I.}, article_number = {2}, doi = {http://dx.doi.org/10.1021/cg1013363}, keywords = {Crystallization; X-ray;Haloalkane dehalogenases}, language = {eng}, issn = {1528-7483}, journal = {Crystal Growth & Design}, title = {Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94}, volume = {11}, year = {2011} }
TY - JOUR ID - 947493 AU - Prudnikova, Tanyana - Chaloupková, Radka - Sato, Yukari - Nagata, Yuji - Degtjarik, O. - Kutý, Michal - Rezacova, P. - Damborský, Jiří - Kuta-Smatanová, Smatanova I. PY - 2011 TI - Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94 JF - Crystal Growth & Design VL - 11 IS - 2 SP - 516-519 EP - 516-519 SN - 15287483 KW - Crystallization KW - X-ray;Haloalkane dehalogenases N2 - The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane. ER -
PRUDNIKOVA, Tanyana, Radka CHALOUPKOVÁ, Yukari SATO, Yuji NAGATA, O. DEGTJARIK, Michal KUTÝ, P. REZACOVA, Jiří DAMBORSKÝ and Smatanova I. KUTA-SMATANOVÁ. Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94. \textit{Crystal Growth \&{} Design}. 2011, vol.~11, No~2, p.~516-519. ISSN~1528-7483. Available from: https://dx.doi.org/10.1021/cg1013363.
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