Substrate Specificity of Haloalkane Dehalogenases

KOUDELÁKOVÁ, Táňa, Eva CHOVANCOVÁ, Jan BREZOVSKÝ, Marta MONINCOVÁ, Andrea FOŘTOVÁ, Jiří JARKOVSKÝ and Jiří DAMBORSKÝ. Substrate Specificity of Haloalkane Dehalogenases. Biochemical Journal. 2011, vol. 435, No 435, p. 345-354. ISSN 0264-6021. Available from: https://dx.doi.org/10.1042/BJ20101405.

Basic information

• Original name: Substrate Specificity of Haloalkane Dehalogenases
• Name in Czech: Substrátová specifita haloalkan dehalogenáz
• Authors: KOUDELÁKOVÁ, Táňa (203 Czech Republic, belonging to the institution), Eva CHOVANCOVÁ (203 Czech Republic, belonging to the institution), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Marta MONINCOVÁ (203 Czech Republic), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Biochemical Journal, 2011, 0264-6021.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.897
• RIV identification code: RIV/00216224:14310/11:00049439
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1042/BJ20101405
• UT WoS: 000289879700005
• Keywords in English: substrate specificity; haloalkane dehalogenases; halogenated compounds
• Tags: AKR, rivok

Abstract

An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• GA203/08/0114, research and development project: Name: Specifické iontové efekty pro proteiny v roztocích a podobné biologicky relevantní systémy.

Investor: Czech Science Foundation, Specific ion effects for proteins in solutions and related biologically relevant systems

• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment