J 2011

Substrate Specificity of Haloalkane Dehalogenases

KOUDELÁKOVÁ, Táňa, Eva CHOVANCOVÁ, Jan BREZOVSKÝ, Marta MONINCOVÁ, Andrea FOŘTOVÁ et. al.

Basic information

Original name

Substrate Specificity of Haloalkane Dehalogenases

Name in Czech

Substrátová specifita haloalkan dehalogenáz

Authors

KOUDELÁKOVÁ, Táňa (203 Czech Republic, belonging to the institution), Eva CHOVANCOVÁ (203 Czech Republic, belonging to the institution), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Marta MONINCOVÁ (203 Czech Republic), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biochemical Journal, 2011, 0264-6021

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.897

RIV identification code

RIV/00216224:14310/11:00049439

Organization unit

Faculty of Science

DOI

UT WoS

000289879700005

Keywords in English

substrate specificity; haloalkane dehalogenases; halogenated compounds

Tags

Změněno: 20/4/2012 10:34, Ing. Andrea Mikešková

Abstract

V originále

An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds.

Links

ED0001/01/01, research and development project
Name: CETOCOEN
GA203/08/0114, research and development project
Name: Specifické iontové efekty pro proteiny v roztocích a podobné biologicky relevantní systémy.
Investor: Czech Science Foundation, Specific ion effects for proteins in solutions and related biologically relevant systems
IAA401630901, research and development project
Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami
Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds
LC06010, research and development project
Name: Centrum biokatalýzy a biotransformací
Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment