Crystallization and Preliminary X-ray Diffraction Analysis of the Wild-Type Haloalkane Dehalogenase DhaA and its Variant DhaA13 Complexed with Different Ligands

STSIAPANAVA, Alena, Radka CHALOUPKOVÁ, Andrea FOŘTOVÁ, J. BRYNDA, M. S. WEISS, Jiří DAMBORSKÝ and Ivana KUTÁ-SMATANOVÁ. Crystallization and Preliminary X-ray Diffraction Analysis of the Wild-Type Haloalkane Dehalogenase DhaA and its Variant DhaA13 Complexed with Different Ligands. Acta Crystallographica F. 2011, vol. 67, No 67, p. 253-257. ISSN 1744-3091. Available from: https://dx.doi.org/10.1107/S1744309110051286.

Basic information

• Original name: Crystallization and Preliminary X-ray Diffraction Analysis of the Wild-Type Haloalkane Dehalogenase DhaA and its Variant DhaA13 Complexed with Different Ligands
• Authors: STSIAPANAVA, Alena (112 Belarus, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), J. BRYNDA (203 Czech Republic), M. S. WEISS (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Ivana KUTÁ-SMATANOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Acta Crystallographica F, 2011, 1744-3091.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.506
• RIV identification code: RIV/00216224:14310/11:00049440
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1107/S1744309110051286
• UT WoS: 000287030600018
• Keywords in English: Crystallization; X-ray Diffraction Analysis; Haloalkane Dehalogenase DhaA
• Tags: AKR, rivok

Abstract

Haloalkane dehalogenases make up an important class of hydrolytic enzymes which catalyse the cleavage of carbon-halogen bonds in halogenated aliphatic compounds. There is growing interest in these enzymes owing to their potential use in environmental and industrial applications. The haloalkane dehalogenase DhaA fromRhodococcus rhodochrous NCIMB 13064 can slowly detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Structural analysis of this enzyme complexed with target ligands was conducted in order to obtain detailed information about the structural limitations of its catalytic properties. In this study, the crystallization and preliminary X-ray analysis of complexes of wild-type DhaA with 2-propanol and with TCP and of complexes of the catalytically inactive variant DhaA13 with the dye coumarin and with TCP are described.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment