Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

LAHODA, M., Radka CHALOUPKOVÁ, Alena STSIAPANAVA, Jiří DAMBORSKÝ and I. SMATANOVA-KUTA. Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane. Acta Crystallographica F. 2011, vol. 67, No 67, p. 397-400. ISSN 1744-3091. Available from: https://dx.doi.org/10.1107/S1744309111001254.

Basic information

• Original name: Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane
• Authors: LAHODA, M. (112 Belarus), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Alena STSIAPANAVA (100 Bulgaria, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and I. SMATANOVA-KUTA (203 Czech Republic).
• Edition: Acta Crystallographica F, 2011, 1744-3091.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 0.506
• RIV identification code: RIV/00216224:14310/11:00049447
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1107/S1744309111001254
• UT WoS: 000288225100022
• Keywords in English: Crystallization; Rhodococcus rhodochrous; DhaA Mutant
• Tags: AKR, rivok

Abstract

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)