J 2011

Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.

HASAN, Khomaini, Andrea FOŘTOVÁ, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, M. ISHITSUKA et. al.

Basic information

Original name

Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.

Authors

HASAN, Khomaini (360 Indonesia, belonging to the institution), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), M. ISHITSUKA (392 Japan), Yuji NAGATA (392 Japan, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution)

Edition

Applied and Environmental Microbiology, 2011, 0099-2240

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.829

RIV identification code

RIV/00216224:14310/11:00049448

Organization unit

Faculty of Science

DOI

UT WoS

000287700100043

Keywords in English

Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58

Tags

Změněno: 20/4/2012 10:38, Ing. Andrea Mikešková

Abstract

V originále

Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.

Links

ED0001/01/01, research and development project
Name: CETOCOEN
IAA401630901, research and development project
Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami
Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds
LC06010, research and development project
Name: Centrum biokatalýzy a biotransformací
Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)