Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.

HASAN, Khomaini, Andrea FOŘTOVÁ, Táňa KOUDELÁKOVÁ, Radka CHALOUPKOVÁ, M. ISHITSUKA, Yuji NAGATA, Jiří DAMBORSKÝ and Zbyněk PROKOP. Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58. Applied and Environmental Microbiology. 2011, vol. 77, No 5, p. 1881-1884. ISSN 0099-2240. Available from: https://dx.doi.org/10.1128/AEM.02109-10.

Basic information

• Original name: Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
• Authors: HASAN, Khomaini (360 Indonesia, belonging to the institution), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), M. ISHITSUKA (392 Japan), Yuji NAGATA (392 Japan, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution).
• Edition: Applied and Environmental Microbiology, 2011, 0099-2240.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.829
• RIV identification code: RIV/00216224:14310/11:00049448
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1128/AEM.02109-10
• UT WoS: 000287700100043
• Keywords in English: Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58
• Tags: AKR, rivok

Abstract

Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.

Links

• ED0001/01/01, research and development project: Name: CETOCOEN
• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)