| NOVÁČEK, Jiří, Anna ZAWADZKA-KAZIMIERCZUK, Veronika PAPOUŠKOVÁ, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI a Vladimír SKLENÁŘ. 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion. Journal of Biomolecular NMR. Dordrecht: Springer Netherlands, roč. 50, č. 1, s. 1-11. ISSN 0925-2738. doi:10.1007/s10858-011-9496-2. 2011. |
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@article{948801,
author = {Nováček, Jiří and ZawadzkaandKazimierczuk, Anna and Papoušková, Veronika and Žídek, Lukáš and Šanderová, Hana and Krásný, Libor and Kozminski, Wiktor and Sklenář, Vladimír},
article_location = {Dordrecht},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s10858-011-9496-2},
keywords = {Intrinsically disordered proteins ; Non-uniform sampling ; 13C detection ; Longitudinal relaxation optimization ; Backbone assignment},
language = {eng},
issn = {0925-2738},
journal = {Journal of Biomolecular NMR},
title = {5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion},
volume = {50},
year = {2011}
}
TY - JOUR
ID - 948801
AU - Nováček, Jiří - Zawadzka-Kazimierczuk, Anna - Papoušková, Veronika - Žídek, Lukáš - Šanderová, Hana - Krásný, Libor - Kozminski, Wiktor - Sklenář, Vladimír
PY - 2011
TI - 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion
JF - Journal of Biomolecular NMR
VL - 50
IS - 1
SP - 1-11
EP - 1-11
PB - Springer Netherlands
SN - 09252738
KW - Intrinsically disordered proteins
KW - Non-uniform sampling
KW - 13C detection
KW - Longitudinal relaxation optimization
KW - Backbone assignment
N2 - Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguous assignment of the disordered part of the protein from a single 5D spectrum.
ER -
NOVÁČEK, Jiří, Anna ZAWADZKA-KAZIMIERCZUK, Veronika PAPOUŠKOVÁ, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI a Vladimír SKLENÁŘ. 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion. \textit{Journal of Biomolecular NMR}. Dordrecht: Springer Netherlands, roč.~50, č.~1, s.~1-11. ISSN~0925-2738. doi:10.1007/s10858-011-9496-2. 2011.
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