Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve

JANČÁLEK, Radim, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve. Neuroscience Letters. Elsevier Ireland Ltd., 2011, vol. 501, No 2, p. 86-91. ISSN 0304-3940. Available from: https://dx.doi.org/10.1016/j.neulet.2011.06.052.

Basic information

• Original name: Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve
• Authors: JANČÁLEK, Radim (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Neuroscience Letters, Elsevier Ireland Ltd. 2011, 0304-3940.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.105
• RIV identification code: RIV/00216224:14740/11:00053053
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.neulet.2011.06.052
• UT WoS: 000294317600006
• Keywords in English: Nerve injury; Cytokines; Neuroinflammation; Sham operation
• Tags: ok, rivok
• Tags: International impact

Abstract

Interleukin-10 prevents transition of a physiological inflammatory reaction to a pathological state that may result in neuropathic pain. We studied bilateral changes of IL-10 in L4–L5 and C7–C8 dorsal root ganglia (DRG) after a chronic constriction injury (CCI) of spinal nerves or the sciatic nerve. Rats were left to survive for 1,3,7 or 14d, sham-operated for 3 or 14 d. IL-10 was detected by immunohistochemical staining and measured using ELISA analysis. Unilateral CCI induced a transient bilateral elevation in IL-10 not only in the homonymous lumbar but also in the heteronymous cervical DRG. Sham operations also induced bilateral elevation of IL-10 in both homonymous and heteronymous DRG. Our experiments revealed that the more proximal is a nerve injury the more rapid is the initial increase and slower the subsequent decrease of IL-10 in DRG. Changes of IL-10 in nonassociated DRG could be related to a general neuroinflammatory reaction of the nervous system to injury and thereby promote potential of the DRG neurons for regenerating their axons following a conditioning lesion.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.