Bilateral changes of IL-10 protein in lumbar and cervical
dorsal root ganglia following proximal and distal chronic
constriction injury of peripheral nerve

J 2011

Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve

JANČÁLEK, Radim, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ

Basic information

Original name

Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve

Authors

JANČÁLEK, Radim (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Neuroscience Letters, Elsevier Ireland Ltd. 2011, 0304-3940

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.105

RIV identification code

RIV/00216224:14740/11:00053053

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.neulet.2011.06.052

UT WoS

000294317600006

Keywords in English

Nerve injury; Cytokines; Neuroinflammation; Sham operation

Abstract

V originále

Interleukin-10 prevents transition of a physiological inflammatory reaction to a pathological state that may result in neuropathic pain. We studied bilateral changes of IL-10 in L4–L5 and C7–C8 dorsal root ganglia (DRG) after a chronic constriction injury (CCI) of spinal nerves or the sciatic nerve. Rats were left to survive for 1,3,7 or 14d, sham-operated for 3 or 14 d. IL-10 was detected by immunohistochemical staining and measured using ELISA analysis. Unilateral CCI induced a transient bilateral elevation in IL-10 not only in the homonymous lumbar but also in the heteronymous cervical DRG. Sham operations also induced bilateral elevation of IL-10 in both homonymous and heteronymous DRG. Our experiments revealed that the more proximal is a nerve injury the more rapid is the initial increase and slower the subsequent decrease of IL-10 in DRG. Changes of IL-10 in nonassociated DRG could be related to a general neuroinflammatory reaction of the nervous system to injury and thereby promote potential of the DRG neurons for regenerating their axons following a conditioning lesion.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

MSM0021622404, plan (intention)

Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.

Files attached

IL10NSL28174.pdf

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Citovat

JANČÁLEK, Radim, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve. Neuroscience Letters. Elsevier Ireland Ltd., 2011, vol. 501, No 2, p. 86-91. ISSN 0304-3940. Available from: https://dx.doi.org/10.1016/j.neulet.2011.06.052.

@article{949872,
   author = {Jančálek, Radim and Hradilová Svíženská, Ivana and Klusáková, Ilona and Dubový, Petr},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.neulet.2011.06.052},
   keywords = {Nerve injury; Cytokines; Neuroinflammation; Sham operation},
   language = {eng},
   issn = {0304-3940},
   journal = {Neuroscience Letters},
   title = {Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve},
   volume = {501},
   year = {2011}
}

TY  - JOUR
ID  - 949872
AU  - Jančálek, Radim - Hradilová Svíženská, Ivana - Klusáková, Ilona - Dubový, Petr
PY  - 2011
TI  - Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve
JF  - Neuroscience Letters
VL  - 501
IS  - 2
SP  - 86-91
EP  - 86-91
PB  - Elsevier Ireland Ltd.
SN  - 03043940
KW  - Nerve injury
KW  - Cytokines
KW  - Neuroinflammation
KW  - Sham operation
N2  - Interleukin-10 prevents transition of a physiological inflammatory reaction to a pathological state that may result in neuropathic pain. We studied bilateral changes of IL-10 in L4–L5 and C7–C8 dorsal root ganglia (DRG) after a chronic constriction injury (CCI) of spinal nerves or the sciatic nerve. Rats were left to survive for 1,3,7 or 14d, sham-operated for 3 or 14 d. IL-10 was detected by immunohistochemical staining and measured using ELISA analysis. Unilateral CCI induced a transient bilateral elevation in IL-10 not only in the homonymous lumbar but also in the heteronymous cervical DRG. Sham operations also induced bilateral elevation of IL-10 in both homonymous and heteronymous DRG. Our experiments revealed that the more proximal is a nerve injury the more rapid is the initial increase and slower the subsequent decrease of IL-10 in DRG. Changes of IL-10 in nonassociated DRG could be related to a general neuroinflammatory reaction of the nervous system to injury and thereby promote potential of the DRG neurons for regenerating their axons following a conditioning lesion.
ER  -

JANČÁLEK, Radim, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral changes of IL-10 protein in lumbar and cervical dorsal root ganglia following proximal and distal chronic constriction injury of peripheral nerve. \textit{Neuroscience Letters}. Elsevier Ireland Ltd., 2011, vol.~501, No~2, p.~86-91. ISSN~0304-3940. Available from: https://dx.doi.org/10.1016/j.neulet.2011.06.052.

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