Endoneurial extracellular matrix influences regeneration and maturation of motor nerve axons—A model of acellular nerve graft

KUBEK, Tomáš, Nabil Ahmed GHALIB and Petr DUBOVÝ. Endoneurial extracellular matrix influences regeneration and maturation of motor nerve axons—A model of acellular nerve graft. Neuroscience Letters. 2011, vol. 496, No 2, p. 75-79. ISSN 0304-3940. doi:10.1016/j.neulet.2011.03.085.

Basic information

• Original name: Endoneurial extracellular matrix influences regeneration and maturation of motor nerve axons—A model of acellular nerve graft
• Authors: KUBEK, Tomáš (203 Czech Republic, belonging to the institution), Nabil Ahmed GHALIB (887 Yemen, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Neuroscience Letters, 2011, 0304-3940.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.105
• RIV identification code: RIV/00216224:14110/11:00053054
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1016/j.neulet.2011.03.085
• UT WoS: 000291196800003
• Keywords in English: Acellular nerve graft; Extracellular matrix; Nerve regeneration
• Tags: International impact

Abstract

Clinical results of functional reinnervation after application of autogeneous nerve grafts obtained from cutaneous nerves have not always been satisfactory.Aforeign extracellular condition especially for regeneration of motor axons is assumed to be one of the reasons explaining these unsuccessful results. The role of endoneurial extracellular matrix in regeneration and maturation of motor axons was studied using acellular nerve segment prepared from muscular or cutaneous nerves applied between stumps of transected motor branch of the femoral nerve. No differences were found in the numbers of regenerated axons and related motoneurons through the motor and cutaneous nerve grafts 1 month after operation. Two months from grafting, however, the numbers of motoneurons and regenerated axons were increased significantly in the motor grafts while these were decreased after regeneration through the cutaneous grafts compared with 1 month. Axons’ diameter and thickness of their myelin sheaths were similar in the cutaneous grafts 1 and 2 months after grafting. In comparison to 1 month, axons had larger diameter and thicker myelin in the motor than cutaneous nerve grafts 2 months from their application. Results of morphometric analysis indicate more beneficial extracellular conditions for regeneration and maturation of myelinated motor nerve axons in the acellular motor than cutaneous nerve graft. Generally, the results revealed that the endoneurial extracellular matrix of motor fibers has a positive effect on regeneration and maturation of motor nerve axons after lesion.

Links

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.