CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ and Michal POHANKA. Digital holographic microscopy in human sperm imaging. Journal of Assisted Reproduction and Genetics. 2011, vol. 28, No 8, p. 725-729. ISSN 1058-0468. Available from: https://dx.doi.org/10.1007/s10815-011-9584-y.
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Basic information
Original name Digital holographic microscopy in human sperm imaging
Authors CRHA, Igor (203 Czech Republic, guarantor, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Martin HUSER (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution), Eva LOUSOVÁ (203 Czech Republic) and Michal POHANKA (203 Czech Republic, belonging to the institution).
Edition Journal of Assisted Reproduction and Genetics, 2011, 1058-0468.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30214 Obstetrics and gynaecology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.844
RIV identification code RIV/00216224:14110/11:00056979
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s10815-011-9584-y
UT WoS 000294734900011
Keywords in English Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 23/3/2012 13:39.
Abstract
The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
Links
NS9661, research and development projectName: Vliv metabolismu homocysteinu na poruchy plodnosti.
Investor: Ministry of Health of the CR
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