Digital holographic microscopy in human sperm imaging

J 2011

Digital holographic microscopy in human sperm imaging

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ et. al.

Basic information

Original name

Digital holographic microscopy in human sperm imaging

Authors

CRHA, Igor (203 Czech Republic, guarantor, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Martin HUSER (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution), Eva LOUSOVÁ (203 Czech Republic) and Michal POHANKA (203 Czech Republic, belonging to the institution)

Edition

Journal of Assisted Reproduction and Genetics, 2011, 1058-0468

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.844

RIV identification code

RIV/00216224:14110/11:00056979

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10815-011-9584-y

UT WoS

000294734900011

Keywords in English

Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity

Abstract

V originále

The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.

Links

NS9661, research and development project

Name: Vliv metabolismu homocysteinu na poruchy plodnosti.

Investor: Ministry of Health of the CR

Citovat

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ and Michal POHANKA. Digital holographic microscopy in human sperm imaging. Journal of Assisted Reproduction and Genetics. 2011, vol. 28, No 8, p. 725-729. ISSN 1058-0468. Available from: https://dx.doi.org/10.1007/s10815-011-9584-y.

@article{950024,
   author = {Crha, Igor and Žáková, Jana and Huser, Martin and Ventruba, Pavel and Lousová, Eva and Pohanka, Michal},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s10815-011-9584-y},
   keywords = {Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity},
   language = {eng},
   issn = {1058-0468},
   journal = {Journal of Assisted Reproduction and Genetics},
   title = {Digital holographic microscopy in human sperm imaging},
   volume = {28},
   year = {2011}
}

TY  - JOUR
ID  - 950024
AU  - Crha, Igor - Žáková, Jana - Huser, Martin - Ventruba, Pavel - Lousová, Eva - Pohanka, Michal
PY  - 2011
TI  - Digital holographic microscopy in human sperm imaging
JF  - Journal of Assisted Reproduction and Genetics
VL  - 28
IS  - 8
SP  - 725-729
EP  - 725-729
SN  - 10580468
KW  - Digital holographic microscopy
KW  - Sperm imaging
KW  - Spermatozoon
KW  - Male infertility
KW  - Chromatin integrity
N2  - The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
ER  -

CRHA, Igor, Jana ŽÁKOVÁ, Martin HUSER, Pavel VENTRUBA, Eva LOUSOVÁ and Michal POHANKA. Digital holographic microscopy in human sperm imaging. \textit{Journal of Assisted Reproduction and Genetics}. 2011, vol.~28, No~8, p.~725-729. ISSN~1058-0468. Available from: https://dx.doi.org/10.1007/s10815-011-9584-y.

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