Molecular detection of Treponema pallidum ssp. pallidum in
clinical samples: sequencing of TP0136, TP0548 and 23S rRNA
genes.

a 2011

Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes.

MIKALOVÁ, Lenka, Petra POSPÍŠILOVÁ, Magdalena FLASAROVÁ, H ZÁKOUCKÁ, Vladana WOZNICOVÁ et. al.

Basic information

Original name

Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes.

Authors

MIKALOVÁ, Lenka, Petra POSPÍŠILOVÁ, Magdalena FLASAROVÁ, H ZÁKOUCKÁ, Vladana WOZNICOVÁ and David ŠMAJS

Edition

International Union of Microbiological Societies 2011 Congress, 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Keywords (in Czech)

Treponema pallidum, klinické vzorky, sekvenování

Změněno: 15/9/2011 13:01, Mgr. Lenka Paštěková, Ph.D.

Abstract

V originále

Syphilis, caused by the spirochete Treponema pallidum subsp. pallidum (TPA), is a sexually transmitted infectious disease with worldwide occurrence. During the years 2004 - 2010, 119 PCR positive specimens from 91 patients (76 males with median age of 33 years and 15 females with median age of 28 years) were collected in the Czech Republic. Treponemal DNA was detected in 4 types of clinical material: genitoanal and pharyngeal swabs (76 samples), whole blood samples (41), cerebrospinal fluid (1) and blood serum (1), especially in patients with primary (60 patients) and secondary (20 patients) stage of syphilis. Primary screening of clinical specimens included nested PCR detection of two TPA specific loci (tmpC and polA genes). To further characterize the treponemal strains, two sequentially variable genes including TP0136 and TP0548 were amplified and sequenced together with the 23S rRNA gene. Altogether, 64.7% of TPA strains were completely typed and 9 treponemal subtypes were identified among 91 tested patients. Mutations in the 23S rRNA gene (A2058G and A2059G), causing macrolide resistance of TPA strains, were found in samples taken from 28.6% of patients. Identified subtypes of TPA strains were further typed with the CDC typing system for TPA treponemes (comprising analysis of the arp and tpr genes). The obtained unique TP0136 and TP0548 sequences were found to combine independently with CDC subtypes indicating their potential for more detailed genetic characterization of TPA-containing clinical samples.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NT11159, research and development project

Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR

Citovat

MIKALOVÁ, Lenka, Petra POSPÍŠILOVÁ, Magdalena FLASAROVÁ, H ZÁKOUCKÁ, Vladana WOZNICOVÁ and David ŠMAJS. Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes. In International Union of Microbiological Societies 2011 Congress. 2011.

@proceedings{950126,
   author = {Mikalová, Lenka and Pospíšilová, Petra and Flasarová, Magdalena and Zákoucká, H and Woznicová, Vladana and Šmajs, David},
   booktitle = {International Union of Microbiological Societies 2011 Congress},
   language = {eng},
   title = {Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes.},
   year = {2011}
}

TY  - CONF
ID  - 950126
AU  - Mikalová, Lenka - Pospíšilová, Petra - Flasarová, Magdalena - Zákoucká, H - Woznicová, Vladana - Šmajs, David
PY  - 2011
TI  - Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes.
N2  - Syphilis, caused by the spirochete Treponema pallidum subsp. pallidum (TPA), is a sexually transmitted infectious disease with worldwide occurrence. During the years 2004 - 2010, 119 PCR positive specimens from 91 patients (76 males with median age of 33 years and 15 females with median age of 28 years) were collected in the Czech Republic. Treponemal DNA was detected in 4 types of clinical material: genitoanal and pharyngeal swabs (76 samples), whole blood samples (41), cerebrospinal fluid (1) and blood serum (1), especially in patients with primary (60 patients) and secondary (20 patients) stage of syphilis. Primary screening of clinical specimens included nested PCR detection of two TPA specific loci (tmpC and polA genes). To further characterize the treponemal strains, two sequentially variable genes including TP0136 and TP0548 were amplified and sequenced together with the 23S rRNA gene. Altogether, 64.7% of TPA strains were completely typed and 9 treponemal subtypes were identified among 91 tested patients. Mutations in the 23S rRNA gene (A2058G and A2059G), causing macrolide resistance of TPA strains, were found in samples taken from 28.6% of patients. Identified subtypes of TPA strains were further typed with the CDC typing system for TPA treponemes (comprising analysis of the arp and tpr genes). The obtained unique TP0136 and TP0548 sequences were found to combine independently with CDC subtypes indicating their potential for more detailed genetic characterization of TPA-containing clinical samples.
ER  -

MIKALOVÁ, Lenka, Petra POSPÍŠILOVÁ, Magdalena FLASAROVÁ, H ZÁKOUCKÁ, Vladana WOZNICOVÁ and David ŠMAJS. Molecular detection of Treponema pallidum ssp. pallidum in clinical samples: sequencing of TP0136, TP0548 and 23S rRNA genes. In \textit{International Union of Microbiological Societies 2011 Congress}. 2011.

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