Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER and Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. Rev. Sci. Instrum. USA: American Institute of Physica, vol. 82, No 9, p. "093701-1"-"093701-8", 8 pp. ISSN 0034-6748. 2011.

Basic information

• Original name: Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy
• Authors: STAIER, Florian (276 Germany), Heinz EIPEL (276 Germany), Petr MATULA (203 Czech Republic, belonging to the institution), Alexei V EVSIKOV (643 Russian Federation), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Christoph CREMER (276 Germany) and Michael HAUSMANN (276 Germany).
• Edition: Rev. Sci. Instrum. USA, American Institute of Physica, 2011, 0034-6748.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.367
• RIV identification code: RIV/00216224:14330/11:00053149
• Organization unit: Faculty of Informatics
• UT WoS: 000295621100023
• Keywords in English: fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors
• Tags: cbia-web
• Tags: International impact, Reviewed

Abstract

To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.

Links

• MSM0021622419, plan (intention): Name: Vysoce paralelní a distribuované výpočetní systémy

Investor: Ministry of Education, Youth and Sports of the CR, Highly Parallel and Distributed Computing Systems

• 2B06052, research and development project: Name: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)

Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images