Micro axial tomography: A miniaturized, versatile stage device
to overcome resolution anisotropy in fluorescence light
microscopy

J 2011

Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK et. al.

Basic information

Original name

Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

Authors

STAIER, Florian (276 Germany), Heinz EIPEL (276 Germany), Petr MATULA (203 Czech Republic, belonging to the institution), Alexei V EVSIKOV (643 Russian Federation), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Christoph CREMER (276 Germany) and Michael HAUSMANN (276 Germany)

Edition

Rev. Sci. Instrum. USA, American Institute of Physica, 2011, 0034-6748

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.367

RIV identification code

RIV/00216224:14330/11:00053149

Organization unit

Faculty of Informatics

UT WoS

000295621100023

Keywords in English

fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors

Abstract

V originále

To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.

Links

MSM0021622419, plan (intention)

Name: Vysoce paralelní a distribuované výpočetní systémy

Investor: Ministry of Education, Youth and Sports of the CR, Highly Parallel and Distributed Computing Systems

2B06052, research and development project

Name: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)

Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images

Citovat

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER and Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. Rev. Sci. Instrum. USA: American Institute of Physica, 2011, vol. 82, No 9, p. "093701-1"-"093701-8", 8 pp. ISSN 0034-6748.

@article{950311,
   author = {Staier, Florian and Eipel, Heinz and Matula, Petr and Evsikov, Alexei V and Kozubek, Michal and Cremer, Christoph and Hausmann, Michael},
   article_location = {USA},
   article_number = {9},
   keywords = {fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors},
   language = {eng},
   issn = {0034-6748},
   journal = {Rev. Sci. Instrum.},
   title = {Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy},
   volume = {82},
   year = {2011}
}

TY  - JOUR
ID  - 950311
AU  - Staier, Florian - Eipel, Heinz - Matula, Petr - Evsikov, Alexei V - Kozubek, Michal - Cremer, Christoph - Hausmann, Michael
PY  - 2011
TI  - Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy
JF  - Rev. Sci. Instrum.
VL  - 82
IS  - 9
SP  - "093701-1"-"093701-8"
EP  - "093701-1"-"093701-8"
PB  - American Institute of Physica
SN  - 00346748
KW  - fluorescence
KW  - glass fibres
KW  - image reconstruction
KW  - image resolution
KW  - optical microscopes
KW  - optical microscopy
KW  - optical tomography
KW  - stepping motors
N2  - To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.
ER  -

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER and Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. \textit{Rev. Sci. Instrum.}. USA: American Institute of Physica, 2011, vol.~82, No~9, p.~''093701-1''-''093701-8'', 8 pp. ISSN~0034-6748.

Detailed Information on Publication Record