VAŘECHA, Miroslav, Michaela POTĚŠILOVÁ, Pavel MATULA and Michal KOZUBEK. Endonuclease G interacts with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET analysis of living cells. In 19th Euroconference on Apoptosis - Metabolism, Epigenetics and Death. 2011.
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Basic information
Original name Endonuclease G interacts with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET analysis of living cells
Name in Czech Endonukleáza G interaguje s histonem H2B, AIF a DNA topoizomerázou II alfa během apoptózy
Authors VAŘECHA, Miroslav (203 Czech Republic, guarantor, belonging to the institution), Michaela POTĚŠILOVÁ (203 Czech Republic, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution) and Michal KOZUBEK (203 Czech Republic, belonging to the institution).
Edition 19th Euroconference on Apoptosis - Metabolism, Epigenetics and Death, 2011.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher Sweden
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14330/11:00053157
Organization unit Faculty of Informatics
Keywords (in Czech) endonukleáza G; histon 2B; DNA topoizomeráza; apoptózu-indukující faktor; mikroskopie živých buněk; FRET.
Keywords in English endonuclease G; histone 2B; DNA topoisomerase; apoptosis-inducing factor; microscopy of living cells; FRET.
Tags International impact
Changed by Changed by: Mgr. Miroslav Vařecha, Ph.D., učo 82780. Changed: 12. 12. 2011 15:06.
Abstract
Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria and then they translocate into the cell nuclei, where they participate in chromatin degradation in a caspase-independent way. The C. elegans homolog of AIF was shown to induce apoptosis and to interact with a homolog of EndoG and together they mediated chromatin DNA degradation. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interact with TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus. These results offer an important insight into the mechanism of apoptotic cell death, which plays a major role in development and progression of degenerative diseases, cancer, and inflammation.
Abstract (in Czech)
Endonukleáza G interaguje s histonem H2B, AIF a DNA topoizomerázou II alfa během apoptózy.
Links
LC535, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii
Investor: Ministry of Education, Youth and Sports of the CR, Basic Research Center
MSM0021622419, plan (intention)Name: Vysoce paralelní a distribuované výpočetní systémy
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
2B06052, research and development projectName: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)
Investor: Ministry of Education, Youth and Sports of the CR, Health and quality of life
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