Endonuclease G interacts with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET analysis of living cells

VAŘECHA, Miroslav, Michaela POTĚŠILOVÁ, Pavel MATULA and Michal KOZUBEK. Endonuclease G interacts with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET analysis of living cells. In 19th Euroconference on Apoptosis - Metabolism, Epigenetics and Death. 2011.

Basic information

• Original name: Endonuclease G interacts with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET analysis of living cells
• Name in Czech: Endonukleáza G interaguje s histonem H2B, AIF a DNA topoizomerázou II alfa během apoptózy
• Authors: VAŘECHA, Miroslav (203 Czech Republic, guarantor, belonging to the institution), Michaela POTĚŠILOVÁ (203 Czech Republic, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution) and Michal KOZUBEK (203 Czech Republic, belonging to the institution).
• Edition: 19th Euroconference on Apoptosis - Metabolism, Epigenetics and Death, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Sweden
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14330/11:00053157
• Organization unit: Faculty of Informatics
• Keywords (in Czech): endonukleáza G; histon 2B; DNA topoizomeráza; apoptózu-indukující faktor; mikroskopie živých buněk; FRET.
• Keywords in English: endonuclease G; histone 2B; DNA topoisomerase; apoptosis-inducing factor; microscopy of living cells; FRET.
• Tags: International impact

Abstract

Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria and then they translocate into the cell nuclei, where they participate in chromatin degradation in a caspase-independent way. The C. elegans homolog of AIF was shown to induce apoptosis and to interact with a homolog of EndoG and together they mediated chromatin DNA degradation. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interact with TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus. These results offer an important insight into the mechanism of apoptotic cell death, which plays a major role in development and progression of degenerative diseases, cancer, and inflammation.

Abstract (in Czech)

Endonukleáza G interaguje s histonem H2B, AIF a DNA topoizomerázou II alfa během apoptózy.

Links

• LC535, research and development project: Name: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Investor: Ministry of Education, Youth and Sports of the CR, Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

• MSM0021622419, plan (intention): Name: Vysoce paralelní a distribuované výpočetní systémy

Investor: Ministry of Education, Youth and Sports of the CR, Highly Parallel and Distributed Computing Systems

• 2B06052, research and development project: Name: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)

Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images